Elucidating the antimicrobial mechanisms of colistin sulfate on Mycobacterium tuberculosis using metabolomics

Tuberculosis
Nadia KoenDu Toit Loots

Abstract

Considering the disadvantageous of first line anti-tuberculosis (TB) drugs, including poor patient adherence, drug side effects, the long treatment duration and rapidly increasing microbe resistance, alternative treatment strategies are needed. Colistin sulfate (CS), a polymyxin antibiotic considered a last-resort antibiotics for treating multidrug-resistant Pseudomonas aeruginosa, Klebsiella pneumoniae, and Acinetobacter, has antimicrobial activity towards mycobacteria, and could serve as a possible anti-TB drug. Using GCxGC-TOFMS metabolomics, we compared the metabolic profiles of Mycobacterium tuberculosis (Mtb) cultured in the presence and absence of CS, to elucidate the mechanisms by which this drug may exert its antimicrobial effects. The principal component analysis of the metabolite data indicated significant variation in the underlying metabolite profiles of the groups. Those metabolites best explaining this differentiation, were acetic acid, and cell wall associated methylated and unmethylated fatty acids, and their alcohol and alkane derivatives. The elevated glucose levels, and various glyoxylate and glycerolipid metabolic intermediates, indicates an elevated flux in these metabolic pathways. Since all the metabolit...Continue Reading

Citations

May 7, 2020·Applied Microbiology and Biotechnology·Adetomiwa A AdenijiDu Toit Loots
Nov 19, 2019·Frontiers in Microbiology·Arshad RizviShekhar C Mande
Mar 27, 2019·Applied Microbiology and Biotechnology·Adetomiwa Ayodele AdenijiOlubukola Oluranti Babalola
May 6, 2020·International Journal of Molecular Sciences·Adel AbouhmadRajni Hatti-Kaul
Oct 19, 2019·Analytical Chemistry·Carolina González-RianoCoral Barbas
Aug 26, 2021·Computational and Structural Biotechnology Journal·Isin T SakalliogluRobert Powers

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