Elucidating the design principles of photosynthetic electron-transfer proteins by site-directed spin labeling EPR spectroscopy

Biochimica Et Biophysica Acta
K Ishara SilvaK V Lakshmi

Abstract

Site-directed spin labeling electron paramagnetic resonance (SDSL EPR) spectroscopy is a powerful tool to determine solvent accessibility, side-chain dynamics, and inter-spin distances at specific sites in biological macromolecules. This information provides important insights into the structure and dynamics of both natural and designed proteins and protein complexes. Here, we discuss the application of SDSL EPR spectroscopy in probing the charge-transfer cofactors in photosynthetic reaction centers (RC) such as photosystem I (PSI) and the bacterial reaction center (bRC). Photosynthetic RCs are large multi-subunit proteins (molecular weight≥300 kDa) that perform light-driven charge transfer reactions in photosynthesis. These reactions are carried out by cofactors that are paramagnetic in one of their oxidation states. This renders the RCs unsuitable for conventional nuclear magnetic resonance spectroscopy investigations. However, the presence of native paramagnetic centers and the ability to covalently attach site-directed spin labels in RCs makes them ideally suited for the application of SDSL EPR spectroscopy. The paramagnetic centers serve as probes of conformational changes, dynamics of subunit assembly, and the relative mo...Continue Reading

References

Jan 1, 1991·Annual Review of Biophysics and Biophysical Chemistry·J Deisenhofer, H Michel
Feb 28, 1989·Biochimica Et Biophysica Acta·K G ParrettJ H Golbeck
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J P AllenD C Rees
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J P AllenD C Rees
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·M D Rabenstein, Y K Shin
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·C AltenbachW L Hubbell
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T F PrisnerR G Griffin
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J H Golbeck
Apr 2, 1998·Biochemistry·Y DeligiannakisA W Rutherford
May 20, 1998·Biophysical Journal·B H McMahonG U Nienhaus
Mar 29, 2001·Nature Structural Biology·W XiaoY K Shin
Apr 6, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·J S Clegg
Apr 28, 2001·Annual Review of Physical Chemistry·T PrisnerF MacMillan
Nov 1, 2001·Biochimica Et Biophysica Acta·P Sétif
Jan 12, 2002·Current Opinion in Structural Biology·K V Lakshmi, G W Brudvig
Jun 19, 2002·Trends in Biochemical Sciences·Linda Columbus, Wayne L Hubbell
Sep 11, 2002·Biochimica Et Biophysica Acta·Pierre SétifJean-David Rochaix
Jan 9, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nobuo Kamiya, Jian-Ren Shen
Mar 12, 2003·Proceedings of the National Academy of Sciences of the United States of America·Zheng FuGary J Gerfen
Aug 9, 2003·Biochimica Et Biophysica Acta·Michael KammelRobert Bittl
Feb 7, 2004·Science·Kristina N FerreiraSo Iwata
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Ben HankamerEgbert J. Boekema
Apr 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Richard H G BaxterJames R Norris

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Current Opinion in Structural Biology
Gail E Fanucci, D S Cafiso
Wiley Interdisciplinary Reviews. RNA
Phuong Nguyen, Peter Z Qin
© 2021 Meta ULC. All rights reserved