Elucidating the identity of Anisakis larvae from a broad range of marine fishes from the Yellow Sea, China, using a combined electrophoretic-sequencing approach

Electrophoresis
Chunxia DuR B Gasser

Abstract

Larval nematodes were collected from marine fishes from the Yellow Sea, China. Specimens (n=1731) of Anisakis type I from 311 fishes (representing 40 species) were each identified based on morphological characters. From the genomic DNA from individual specimens, a region of nuclear ribosomal DNA was amplified by PCR, followed by digestion with restriction endonuclease HinfI, TaqI or HhaI. Subsequently, the ITS-1 and ITS-2 regions of selected samples were sequenced. The results revealed three species of Anisakis, namely Anisakis pegreffii (n=1709), A. typica (n=3) and a genotype (n=19) proposed, also based on comparison with previous studies, to be a "hybrid" between A. pegreffii and A. simplex sensu stricto. Thus, A. pegreffii was the dominant species, accounting for 98.7% of the total number of specimens examined herein. This is the first report of A. typica and the "hybrid" genotype from fishes from the Yellow Sea. This study provides important basic information on Anisakis in this region and suggests that the genus Anisakis has substantial host and geographical distributions.

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Citations

Sep 18, 2012·Parasitology Research·I Vardić SmrzlićE Teskeredžić
Jan 8, 2016·Systematic Parasitology·Liang LiLu-Ping Zhang
Oct 1, 2014·International Journal of Food Microbiology·Tae-Jong BakJeong-Ho Kim
Oct 28, 2011·Veterinary Parasitology·Ilias ChaligiannisSmaragda Sotiraki
Sep 24, 2014·The Korean Journal of Parasitology·Woon-Mok SohnByoung-Kuk Na
Sep 10, 2010·International Journal of Food Microbiology·Azusa UmeharaHiromu Sugiyama
Aug 29, 2016·Parasitology International·Antonella CostaVincenzo Ferrantelli

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