PMID: 9639557Jun 26, 1998Paper

Elucidation of a promoter activity that directs the expression of acetyl-CoA carboxylase alpha with an alternative N-terminus in a tissue-restricted fashion

The Biochemical Journal
M C Barber, M T Travers

Abstract

Previous studies in rats and humans have demonstrated that acetyl-CoA carboxylase alpha (ACC-alpha), the principal ACC isoenzyme in lipogenic tissues, is transcribed from two promoters, PI and PII, that operate in a tissue-specific fashion. Each promoter gives rise to ACC-alpha mRNA isoforms that differ in their 5' untranslated regions but essentially encode the same protein product. In the present study we demonstrate that such a pattern of promoter usage is evident in sheep tissues but in addition we have detected the expression of a novel ACC-alpha mRNA isoform that is expressed in a variety of tissues including kidney, lung, liver and mammary gland, where it is markedly induced during lactation. This novel transcript differs from the previously described ACC-alpha mRNA in that exon 5, the primary coding exon in both PI and PII transcripts, is replaced by a 424-nt sequence that seems to represent the 5' terminus of the mRNA. The 424-nt sequence encodes a 17-residue N-terminal region as the N-terminal residue in the deduced sequence is a methionine flanked by several in-frame stop codons. The 5' terminal 424 nt are present as a single exon, which we have termed exon 5A, in the sheep ACC-alpha gene and this is located approx. ...Continue Reading

Citations

Jun 18, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jianqiang MaoSalih J Wakil
Jun 20, 2013·Animal Biotechnology·María MuñozEstefânia Alves
Apr 27, 2016·Animal : an International Journal of Animal Bioscience·E TicianiD E De Oliveira
Jul 23, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Adrian MolenaarHans-Martin Seyfert
Dec 22, 1999·The Proceedings of the Nutrition Society·R G VernonM T Travers
Nov 9, 2005·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·B MoioliG Catillo
Feb 7, 2001·The Journal of Biological Chemistry·A N BooneR W Brownsey
Oct 27, 2005·The Journal of Biological Chemistry·Weissy M LeeRoger W Brownsey
Jun 25, 2003·Biochemical and Biophysical Research Communications·Andreas Kolb
Mar 8, 2005·Biochimica Et Biophysica Acta·Michael C BarberMaureen T Travers
Jun 13, 2006·Developmental Biology·Jaebok Choi, Anna P Newman

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