Elucidation of apoptosis induced by serum deprivation in cultured conjunctival epithelial cells

The British Journal of Ophthalmology
A HiguchiK Tsubota

Abstract

The conjunctival epithelial cell line, CCL20.2 (CCL), requires the presence of 10% fetal calf serum (FCS) in the medium to survive. To elucidate the molecular mechanism underlying such cell death, including the death signal for these cells, the activities of several caspases in the CCL were measured, and the effects of caspase inhibitors and serum components on cell death were examined. CCL was grown in Medium 199 containing 10% FCS, and the medium was changed to Medium 199 with or without 10% FCS, or medium without 10% FCS but containing caspase inhibitors or serum components. After 24 hours' incubation, the enzyme activities of caspases 1, 3, 8, and 9 in the culture supernatants were measured, and the effects of caspase inhibitors and serum components-for example, growth factors, lactoferrin, retinoic acid, were investigated. DNA fragmentation was induced by serum deprivation, confirming that serum deprivation induces apoptosis in CCL. While the activities of caspases 3 and 8 were found to be increased, those of caspases 1 and 9 were not detected in the apoptotic cells. Z-VAD completely suppressed the caspase 3 activation, and specific inhibitors of caspases 1, 8, and 9 partially suppressed the activation. Serum deprivation i...Continue Reading

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Citations

Jul 10, 2010·Nucleic Acids Research·Kai WangDavid J Galas
Oct 1, 2008·Cornea·Takashi KojimaKazuo Tsubota
Oct 17, 2012·Cornea·Kazuo TsubotaTetsuya Kawakita
Apr 3, 2010·PloS One·Akihiro HiguchiKazuo Tsubota
Jan 23, 2016·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Lin YangXiangyuan Liu
Aug 16, 2011·Matrix Biology : Journal of the International Society for Matrix Biology·Jean-Marie ZahmPhilippe Birembaut
Aug 1, 2006·American Journal of Ophthalmology·Stephen C Pflugfelder
Feb 10, 2016·International Journal of Cancer. Journal International Du Cancer·Hoi Yan NgMaria Li Lung
Oct 28, 2015·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Amaris A GenemarasChun-Yuh Huang
Jan 9, 2015·Chonnam Medical Journal·Kyung Chul Yoon
Nov 28, 2018·Investigative Ophthalmology & Visual Science·Akihiro Higuchi
Mar 17, 2007·Current Eye Research·Akihiro HiguchiKazuo Tsubota

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