Elucidation of Biochemical Pathways Underlying VOCs Production in A549 Cells

Frontiers in Molecular Biosciences
Takeshi FuruhashiShigenori Ota

Abstract

Cellular volatile organic compounds (VOCs) are unique compounds whose metabolic pathways remain enigmatic. To elucidate their metabolism, we investigated the VOCs of lung cancer A549 and 2 non-cancer lung cells (HLB; HBEpC). Neutral sugars and lactate in the medium were measured by colorimetric assay. VOCs were enriched by monotrap and profiled by GC-MS. To investigate the enzymes that change VOC metabolism in cells, we conducted ALDH activity assays and qPCR. ROS (reactive oxygen species) assays were conducted to assess oxidation stress. The colorimetric assay showed that especially A549 and HLB took up sugars from the medium and rapidly secreted lactate into the medium. The VOC profile (GC-MS) revealed a trans-2-hexenol increase, especially in A549 lung cancer cells. This is a novel lipid peroxidation product from animal cells. Based on the absolute quantification data, trans-2-hexenol increased in parallel with number of A549 cancer cells incubated. The qPCR data implies that ADH1c potentially plays an important role in the conversion into trans-2-hexenol.

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Citations

Dec 22, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Kazutoshi Sugita, Hiroshi Sato
Feb 16, 2021·Frontiers in Molecular Biosciences·Andreas LeihererPaweł Mochalski
Feb 10, 2021·Genes·Matteo MartinaArnaud G Bovy

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Methods Mentioned

BETA
FCS
PCR

Software Mentioned

monotrap
Enspire
Mass Hunter
AdvanView

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