Elucidation of IgE-binding epitopes of Ani s 1: the major Anisakis simplex allergen

Molecular and Biochemical Parasitology
Yukihiro KobayashiKazuo Shiomi

Abstract

Ani s 1, the major allergen of Anisakis simplex, is expected to be a useful antigen in allergen-specific immunotherapy of Anisakis allergy. To avoid side-effects such as anaphylactic shock in immunotherapy, it is desirable to use not native allergens but hypoallergenic mutants devoid of IgE-binding epitopes. This study was hence aimed to elucidate IgE-binding epitopes of Ani s 1 toward the development of hypoallergenic Ani s 1 mutants. Mapping experiments using 32 peptides (P1-32) revealed that major IgE-binding epitopes are included in P24 (region 116-130) and P28 (region 136-150). Furthermore, Ala-scanning and truncation experiments established that eight (underlined in the sequence 116-ELFAREYEGVCKSGK-130) and nine amino acid residues (underlined in the sequence 136-RGSGWMMTILGKSCD-150) are crucial for the IgE-binding of P24 and P28, respectively. The determined crucial residues of P24 and P28 were assumed to be involved in electrostatic and hydrophobic interactions with IgE, respectively.

References

Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·S Jones, J M Thornton
Jul 27, 2002·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·María Luisa Caballero, Ignacio Moneo
Aug 4, 2004·Molecular and Biochemical Parasitology·Kuniyoshi ShimakuraKazuo Shiomi
Jun 1, 2007·Molecular and Biochemical Parasitology·Rosa Rodriguez-PerezIgnacio Moneo
Jun 19, 2007·Parasitology Research·Ignacio MoneoMiguel Gonzalez-Muñoz

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Citations

Dec 17, 2014·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·M Garcia AlonsoR Rodríguez-Pérez
Jul 3, 2013·International Archives of Allergy and Immunology·Heidi R MyrsetMaaike M B W Dooper
Dec 18, 2013·Toxicon : Official Journal of the International Society on Toxinology·Salvatore G De-SimoneDavid W Provance

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