Elucidation of interfacial pH behaviour at the cell/substrate nanogap for in situ monitoring of cellular respiration

Nanoscale
Hiroto SatakeToshiya Sakata

Abstract

In situ monitoring of cellular metabolism is useful for elucidating dynamic functions of living cells. In our previous studies, cellular respiration was continuously monitored as a change in pH at the cell/electrode nanoscale interface (i.e., interfacial pH) using an ion-sensitive field-effect transistor (ISFET). However, such interfacial pH behaviour on the nanoscale has not been confirmed using other methods such as fluorescence imaging. In this study, we have clarified the interfacial pH behaviour at a cell/substrate nanogap using a laser scanning confocal fluorescence microscope. The phospholipid fluorescein used as a pH indicator was fixed to the plasma membrane on the external side of a cell by inserting its lipophilic alkyl chain into the membrane, and used to observe the change in interfacial pH. As a result, hydrogen ions generated by cellular respiration were gradually accumulated at the cell/substrate nanogap, resulting in a decrease in pH. Moreover, the interfacial pH between the plasma membrane and the substrate became lower than the pH near the surface of cells not in contact with the substrate. The data obtained in this study support the idea that potentiometric ion sensors such as ISFETs can detect a cellular-me...Continue Reading

References

Jan 1, 1985·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K H Jones, J A Senft
Aug 1, 1993·Human Immunology·X M WangS Hardy
Jan 1, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Takashi IkenoueR Lane Smith
Jun 12, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Stephen D WaldmanRita A Kandel
Jan 30, 2004·Diabetes·J Antonie MaassenHerman H P J Lemkes
Sep 23, 2006·Proteomics·Ingrid MillerElisabetta Gianazza
Feb 22, 2007·Annals of the New York Academy of Sciences·Peter Fromherz
Jan 15, 2008·Journal of Peptide Science : an Official Publication of the European Peptide Society·Pedro M MatosNuno C Santos
Jun 17, 2008·Biochemical and Biophysical Research Communications·H K Heywood, D A Lee
Oct 27, 2009·Biochimica Et Biophysica Acta·Paula I MoreiraGeorge Perry
Aug 28, 2012·Nature Materials·Bozhi TianCharles M Lieber
Jun 20, 2013·Analytical Chemistry·Toshiya SakataHiroaki Inui
May 14, 2016·Circulation Research·Edward J LesnefskyCharles L Hoppel
Aug 28, 2016·Biochimica Et Biophysica Acta·Kazuhiro SakamakiNaoto Ueno
Dec 22, 2016·Biophysical Journal·Talita L SantosAna Paula Valente
Sep 20, 2017·Nature Communications·Douglas S RichardsonStefan W Hell
Nov 9, 2017·Analytical Chemistry·Haoyue YangToshiya Sakata

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Citations

Oct 9, 2019·Science and Technology of Advanced Materials·Kensuke ItoToshiya Sakata
Sep 18, 2020·Science and Technology of Advanced Materials·Toshiya SakataKazuhiko Nishi
Jun 11, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Hiroto Satake, Toshiya Sakata
Sep 21, 2018·ACS Applied Materials & Interfaces·Taira Kajisa, Toshiya Sakata

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Methods Mentioned

BETA
fluorescence microscopy
fluorescence imaging
Fluorescence

Software Mentioned

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