Elucidation of linear epitopes of pertussis toxin using overlapping synthetic decapeptides: identification of a human B-cell determinant in the S1 subunit indicative of acute infections

Microbial Pathogenesis
B Raupach, M A Schmidt

Abstract

To identify relevant linear epitopes within the immunodominant ADP-ribosyl transferase (S1 subunit) of pertussis toxin (PT), its complete amino acid sequence was synthesized as consecutive, overlapping decapeptides on solid phase and probed for seroreactivity with pertussis specific human antisera in 'peptide scans'. Comparison of the resulting antigenic profiles revealed two distinct types of human antisera, though amino acids 140-200 could not be assessed as the corresponding peptides reacted non-specifically with the detection system. Human anti-pertussis sera predominantly recognized linear immunodominant epitopes located in three separated segments spanning amino acids 3-16, 21-30, and 211-222. Antisera originating from infants with acute B. pertussis infections (type I) identified determinants in all three segments, while type-II antisera from convalescent patients only recognized epitopes in the N-terminal regions. The binding of pertussis specific antisera--both type I and type II--to the holotoxin was inhibited by preincubation of antibodies with synthetic peptides corresponding to two linear determinants located at the N-terminus of S1:R 3-16 and R 21-30. However, competitive binding of antibodies to PT and to synthet...Continue Reading

Citations

Jul 25, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A MichilsJ Duchateau
May 21, 1999·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·A MichilsJ Duchateau
Mar 1, 1997·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J DuchateauL Baras
Jul 10, 2010·Middle East African Journal of Ophthalmology·Pelin Kaynak-Hekimhan
Jun 5, 2007·Journal of Clinical Neurophysiology : Official Publication of the American Electroencephalographic Society·Tomokazu ObiKouichi Mizoguchi
Feb 19, 2010·Clinical and Vaccine Immunology : CVI·Katy Beaumont, Fotini Betsou

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