Elucidation of molecular mechanisms of physiological variations between bovine subcutaneous and visceral fat depots under different nutritional regimes

PloS One
Josue Moura RomaoLe Luo Guan

Abstract

Adipose tissue plays a critical role in energy homeostasis and metabolism. There is sparse understanding of the molecular regulation at the protein level of bovine adipose tissues, especially within different fat depots under different nutritional regimes. The objective of this study was to analyze the differences in protein expression between bovine subcutaneous and visceral fat depots in steers fed different diets and to identify the potential regulatory molecular mechanisms of protein expression. Subcutaneous and visceral fat tissues were collected from 16 British-continental steers (15.5 month old) fed a high-fat diet (7.1% fat, n=8) or a control diet (2.7% fat, n=8). Protein expression was profiled using label free quantification LC-MS/MS and expression of selected transcripts was evaluated using qRT-PCR. A total of 682 proteins were characterized and quantified with fat depot having more impact on protein expression, altering the level of 51.0% of the detected proteins, whereas diet affected only 5.3%. Functional analysis revealed that energy production and lipid metabolism were among the main functions associated with differentially expressed proteins between fat depots, with visceral fat being more metabolically active ...Continue Reading

References

Apr 1, 1993·Computer Applications in the Biosciences : CABIOS·M HirosawaT Toya
Aug 15, 2002·Experimental Gerontology·James L KirklandIordanes Karagiannides
Nov 5, 2004·Diabetes & Metabolism·V LargeM Beylot
Jun 28, 2005·Molecular & Cellular Proteomics : MCP·William M OldNatalie G Ahn
Sep 13, 2005·Cell Metabolism·Jun LiuAlan R Saltiel
Dec 2, 2006·Nature Reviews. Molecular Cell Biology·Evan D Rosen, Ormond A MacDougald
Mar 31, 2007·Diabetes·Naomi HosogaiIichiro Shimomura
Dec 7, 2007·Biochemical and Biophysical Research Communications·Masaaki TaniguchiStephen S Moore
Jul 16, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Johanna SamulinHilde Sundvold
Mar 10, 2009·Trends in Endocrinology and Metabolism : TEM·Martina I Lefterova, Mitchell A Lazar
Mar 11, 2009·Nature Reviews. Genetics·Juan M VaquerizasNicholas M Luscombe
Mar 12, 2009·Current Protein & Peptide Science·K M Barnes, J L Miner
Mar 25, 2009·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Y Wang, M A Beydoun
Aug 5, 2009·Expert Opinion on Therapeutic Targets·Huangming XieHarvey F Lodish
Aug 25, 2009·Clinics in Chest Medicine·Victoria A CatenacciHolly R Wyatt
Sep 3, 2009·Molecular and Cellular Endocrinology·Sandra GalicGregory R Steinberg
Sep 8, 2009·Molecular and Cellular Endocrinology·Ursula A White, Jacqueline M Stephens
Sep 18, 2009·BMC Molecular Biology·Weiwu JinLe Luo Guan
Oct 6, 2009·Biochemical and Biophysical Research Communications·Michael KarbienerMarcel Scheideler
Dec 23, 2009·Molecular and Cellular Endocrinology·Sylvia P PoulosGary J Hausman
Jan 12, 2010·Biochemical and Biophysical Research Communications·Sang Yun KimJae Bum Kim
May 25, 2010·Journal of Food Science·Michael V DodsonJames M Reecy
Dec 1, 2008·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Masaaki TaniguchiStephen S Moore
Jul 27, 2010·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Ramanna Valmiki RajeshHyun-Jeong Lee
Nov 13, 2010·Gene Regulation and Systems Biology·Sandeep J JosephSusan K Duckett
May 19, 2011·American Journal of Physiology. Endocrinology and Metabolism·Le ZhangJeffrey N Keller
May 20, 2011·Nature·Björn SchwanhäusserMatthias Selbach
Jun 10, 2011·Nature·Mirko TrajkovskiMarkus Stoffel
Jun 23, 2011·PLoS Genetics·Anatole GhazalpourAldons J Lusis
Jul 9, 2011·Meat Science·Shalene H McNeillMary E Van Elswyk
Aug 17, 2011·Experimental Biology and Medicine·Josue M RomaoLe Luo Guan
Sep 29, 2011·Nature Reviews. Molecular Cell Biology·Ana G Cristancho, Mitchell A Lazar
Jan 10, 2012·Cell Death and Differentiation·D Ferland-McColloughS E Ozanne

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Methods Mentioned

BETA
electrophoresis
Protein Assay
PCR

Software Mentioned

Mascot
Motic Images Plus
Downstream Effects Analysis ( IPA
Progenesis LC - MS
Ingenuity Pathway Analysis Software Package ( IPA
TargetScan
Progenesis LCMS
Ingenuity Pathway Analysis Package ( IPA )
Ingenuity Knowledge Base
Ingenuity Knowledge Base ( IPA

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