Elucidation of structural and functional properties of albumin bound to gold nanoparticles

Journal of Biomolecular Structure & Dynamics
J MariamP M Dongre

Abstract

Nanoparticle-albumin complexes are being designed for targeted drug delivery and imaging. However, the changes in the functional properties of albumin due to adsorption on nanoparticles remain elusive. Thus, the objective of this work was to elucidate the structural and functional properties of human and bovine serum albumin bound to negatively charged gold nanoparticles (GNPs). Fluorescence data demonstrated static quenching of albumin by GNP with the quenching of buried as well as surface tryptophan in BSA. The binding process was enthalpy and entropy-driven in HSA and BSA, respectively. At lower concentrations of GNP there was a higher affinity for tryptophan, whereas at higher concentrations both tryptophan and tyrosine participated in the interaction. Synchronous fluorescence spectra revealed that the microenvironment of tryptophan in HSA turned more hydrophilic upon exposure to GNP. The α-helical content of albumin was unaltered by GNP. Approximately 37 and 23% reduction in specific activity of HSA and BSA was observed due to GNP binding. In presence of warfarin and ibuprofen the binding constants of albumin-GNP complexes were altered. A very interesting observation not reported so far is the retained antioxidant activity...Continue Reading

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Citations

Apr 26, 2016·Journal of Biomolecular Structure & Dynamics·Laxmi N JattinagoudarShivamurti A Chimatadar
Jun 7, 2017·Journal of Biomolecular Structure & Dynamics·Yousuf Sameena, Israel V M V Enoch
Aug 26, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Karen BolañosEyleen Araya
Aug 18, 2018·Frontiers in Immunology·Matthias BrosStephan Grabbe
Jan 30, 2021·Journal of Biomolecular Structure & Dynamics·Manik N WaghmarePrabhakar M Dongre
Jan 22, 2021·The Journal of Physical Chemistry. B·Nurul Ain AzmanJames Chen Yong Kah
Dec 13, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Nurul 'Ain AzmanJames Chen Yong Kah
Jun 19, 2018·The Journal of Physical Chemistry. B·Marta GambucciLoredana Latterini

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Methods Mentioned

BETA
circular dichroism
transmission electron microscopy
surface plasmon resonance
small
angle neutron scattering
Fluorescence
light scattering
fluorescence studies
acetylation
fluorescence spectroscopy

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