PMID: 7581336Sep 1, 1995Paper

Elucidation of the DNA synthetic cycle of Entamoeba spp. using flow cytometry and mathematical modeling

The Journal of Eukaryotic Microbiology
J A DvorakC W Hallahan

Abstract

We developed a method to study the DNA synthetic cycles of Entamoeba histolytica and Entamoeba invadens by flow cytometry (FCM) based on a preparative procedure to reduce both high levels of natural fluorescence and non-specific adsorption of fluorochromes. We modeled G1, S, and G2 phases as a series of overlapping Gaussian curves. Both E. histolytica and E. invadens displayed G1, S, and G2 proportions that are consistent with eukaryotic cell populations in exponential or stationary growth phase. Exponential phase E. histolytica populations contained a hypodiploid subset with a mass of about 20% less than the diploid value which we estimate by FCM to be 24 x 10(-14) g DNA/cell. Exponential phase E. invadens populations contained a hypodiploid subset with a mass of about 6% less than the diploid value which we estimate by FCM to be 30 x 10(-14) g DNA/cell.

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Citations

Apr 3, 2003·International Journal for Parasitology·Stepanka VanacovaPatricia J Johnson
Mar 25, 2000·International Journal for Parasitology·A BhattacharyaS Bhattacharya
Feb 27, 2001·Molecular and Biochemical Parasitology·A Ganguly, A Lohia
Apr 24, 1999·Molecular and Biochemical Parasitology·U Willhoeft, E Tannich
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Aug 21, 2008·PLoS Neglected Tropical Diseases·Chandrama MukherjeeAnuradha Lohia
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Aug 24, 2010·Experimental Parasitology·Nishant SinghJaishree Paul
Feb 11, 2012·Molecular and Biochemical Parasitology·Nishant SinghSudha Bhattacharya
Jan 23, 2016·Scientific Reports·Olga Hernández de la CruzCésar López-Camarillo
Feb 22, 2002·Cellular Microbiology·Suchismita Das, Anuradha Lohia
Jul 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D Eichinger
Jun 27, 2007·Bioscience Reports·Anuradha LohiaPromita Ghosh Dastidar

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