Elucidation of the final reactions of DIMBOA-glucoside biosynthesis in maize: characterization of Bx6 and Bx7

Plant Physiology
Rafal JonczykA Gierl

Abstract

Benzoxazinoids were identified in the early 1960s as secondary metabolites of the grasses that function as natural pesticides and exhibit allelopathic properties. Benzoxazinoids are synthesized in seedlings and stored as glucosides (glcs); the main aglucone moieties are 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one (DIBOA) and 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one (DIMBOA). The genes of DIBOA-glc biosynthesis have previously been isolated and the enzymatic functions characterized. Here, the enzymes for conversion of DIBOA-glc to DIMBOA-glc are identified. DIBOA-glc is the substrate of the dioxygenase BENZOXAZINLESS6 (BX6) and the produced 2,4,7-trihydroxy-2H-1,4-benzoxazin-3-(4H)-one-glc is metabolized by the methyltransferase BX7 to yield DIMBOA-glc. Both enzymes exhibit moderate K(m) values (below 0.4 mm) and k(cat) values of 2.10 s(-1) and 0.25 s(-1), respectively. Although BX6 uses a glucosylated substrate, our localization studies indicate a cytoplasmic localization of the dioxygenase. Bx6 and Bx7 are highest expressed in seedling tissue, a feature shared with the other Bx genes. At present, Bx6 and Bx7 have no close relatives among the members of their respective gene families. Bx6 and Bx7 map to the cluster of ...Continue Reading

References

Aug 1, 1975·The Biochemical Journal·A Cornish-Bowden
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Jan 1, 1995·The Plant Cell·R J BensenS P Briggs
Oct 1, 1996·Phytochemistry·Y Hashimoto, K Shudo
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·D MelansonW S Chilton
Mar 4, 1998·Plant Molecular Biology·A B ChristensenD B Collinge
Aug 1, 1998·Plant Molecular Biology·C P Joshi, V L Chiang
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·K OsakabeV L Chiang
Sep 10, 1999·Journal of Natural Products·A Hofmann, D Sicker
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·D Ober, T Hartmann
Nov 9, 2000·Archives of Biochemistry and Biophysics·D Anzellotti, R K Ibrahim
Feb 13, 2001·The Plant Journal : for Cell and Molecular Biology·K ParvathiR A Dixon
Aug 29, 2001·Phytochemistry·N KatanoK Inoue
Feb 20, 2002·The Plant Journal : for Cell and Molecular Biology·U von RadM Frey
Mar 7, 2003·Phytochemistry·Monika FreyAlfons Gierl
Jun 26, 2003·Nucleic Acids Research·Olivier PoirotCedric Notredame
Oct 14, 2003·Plant Physiology·Mark D Curtis, Ueli Grossniklaus
May 19, 2004·Proceedings of the National Academy of Sciences of the United States of America·X QiA Osbourn
Jun 23, 2004·The Plant Cell·Christopher Dean GoodmanVirginia Walbot
Dec 9, 2004·The Plant Journal : for Cell and Molecular Biology·C Douglas GrubbSteffen Abel
Dec 15, 2004·BMC Plant Biology·Dominique Anzellotti, Ragaï K Ibrahim
Oct 14, 2005·Molecular Biology and Evolution·Daniel H Huson, David Bryant
Nov 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Taiji NomuraHajime Iwamura
Dec 22, 2005·The Plant Journal : for Cell and Molecular Biology·Eiichiro OnoToru Nakayama
Jul 11, 2006·Plant Molecular Biology·Huaijun Michael LiFaith C Belanger
Feb 1, 2007·Annual Review of Plant Biology·Philip A Rea

❮ Previous
Next ❯

Citations

Aug 8, 2009·Cellular and Molecular Life Sciences : CMLS·Anne E Osbourn, Ben Field
Nov 17, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Ana ButrónM D McMullen
May 23, 2012·Journal of Chemical Ecology·Margot SchulzVincenzo Tabaglio
Feb 23, 2012·Plant Physiology·Rita Mozes-KochIlan Sela
Mar 4, 2014·Trends in Plant Science·Svetlana BoychevaTeresa B Fitzpatrick
Feb 20, 2014·The Plant Journal : for Cell and Molecular Biology·Yosuke KawaiMasaharu Mizutani
Jun 12, 2012·Current Opinion in Plant Biology·Daniel J Kliebenstein, Anne Osbourn
May 13, 2008·Phytochemistry·Anne Vinther MorantSøren Bak
Aug 16, 2011·The Plant Journal : for Cell and Molecular Biology·Gaétan GlauserMatthias Erb
Aug 29, 2014·Biological Reviews of the Cambridge Philosophical Society·Stefan PentzoldSøren Bak
Jan 21, 2015·Molecular Nutrition & Food Research·Khem B AdhikariInge S Fomsgaard
Apr 1, 2014·Current Opinion in Biotechnology·Hans-Wilhelm Nützmann, Anne Osbourn
Oct 28, 2014·Frontiers in Plant Science·Scott C Farrow, Peter J Facchini
Apr 27, 2016·The New Phytologist·Hans-Wilhelm NützmannAnne Osbourn
Feb 14, 2006·Journal of Plant Physiology·Riffat JabeenKoji Hasegawa
Mar 23, 2011·Plant Science : an International Journal of Experimental Plant Biology·Masayuki SueShunsuke Yajima
Nov 18, 2016·Bioscience, Biotechnology, and Biochemistry·Yu KokuboAtsushi Ishihara
Jan 22, 2017·The Plant Journal : for Cell and Molecular Biology·Amit RaiMami Yamazaki
Mar 28, 2018·Plant & Cell Physiology·Shaoqun ZhouGeorg Jander
Feb 11, 2015·Journal of Applied Genetics·Beata BakeraMonika Rakoczy-Trojanowska
Nov 26, 2018·The Plant Journal : for Cell and Molecular Biology·Bowen LuoShibin Gao
Mar 30, 2011·The Plant Journal : for Cell and Molecular Biology·Hoi Yee ChuAnne Osbourn
May 10, 2017·Nucleic Acids Research·Nadine TöpferAsaph Aharoni
Apr 13, 2019·Microbiome·Enoch Narh KudjordjieMogens Nicolaisen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.