Elucidation of Xylem-Specific Transcription Factors and Absolute Quantification of Enzymes Regulating Cellulose Biosynthesis in Populus trichocarpa

Journal of Proteome Research
Philip L LoziukDavid C Muddiman

Abstract

Cellulose, the main chemical polymer of wood, is the most abundant polysaccharide in nature.1 The ability to perturb the abundance and structure of cellulose microfibrils is of critical importance to the pulp and paper industry as well as for the textile, wood products, and liquid biofuels industries. Although much has been learned at the transcript level about the biosynthesis of cellulose, a quantitative understanding at the proteome level has yet to be established. The study described herein sought to identify the proteins directly involved in cellulose biosynthesis during wood formation in Populus trichocarpa along with known xylem-specific transcription factors involved in regulating these key proteins. Development of an effective discovery proteomic strategy through a combination of subcellular fractionation of stem differentiating xylem tissue (SDX) with recently optimized FASP digestion protocols, StageTip fractionation, as well as optimized instrument parameters for global proteomic analysis using the quadrupole-orbitrap mass spectrometer resulted in the deepest proteomic coverage of SDX protein from P. trichocarpa with 9,146 protein groups being identified (1% FDR). Of these, 20 cellulosic/hemicellulosic enzymes and 4...Continue Reading

References

Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·J R PearD M Stalker
Feb 21, 1998·Science·T ArioliR E Williamson
Oct 12, 2000·Plant Physiology·T A Richmond, C R Somerville
Apr 20, 2001·Current Opinion in Plant Biology·K Keegstra, N Raikhel
Oct 20, 2001·Current Opinion in Plant Biology·K S Dhugga
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Deborah P. Delmer
May 12, 2004·Current Opinion in Plant Biology·Wolf-Rüdiger Scheible, Markus Pauly
May 29, 2004·Analytical Chemistry·Jan Havlis, Andrej Shevchenko
Apr 30, 2005·Angewandte Chemie·Dieter KlemmAndreas Bohn
Jul 11, 2006·Annual Review of Cell and Developmental Biology·Chris Somerville
Aug 16, 2006·Analytical Chemistry·Barbara E FrewenMichael J MacCoss
Oct 24, 2007·Current Opinion in Plant Biology·Ruiqin Zhong, Zheng-Hua Ye
Apr 21, 2009·Trends in Plant Science·Manoj KumarBrian E Ellis
Apr 21, 2009·Nature Methods·Jacek R WiśniewskiMatthias Mann
Apr 29, 2009·Plant & Cell Physiology·Junko TakahashiEwa J Mellerowicz
Sep 11, 2009·Proteomics·Udaya C KalluriDale A Pelletier
Oct 24, 2009·Journal of Proteome Research·Jacek R WiśniewskiMatthias Mann
Jan 6, 2010·Journal of Proteome Research·Kung-Yen ChangDavid C Muddiman
Jan 14, 2011·Trends in Plant Science·Qiao Zhao, Richard A Dixon
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Genna L AndrewsDavid C Muddiman
Dec 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Hsi-Chuan ChenVincent L Chiang
Oct 9, 2012·Journal of Proteome Research·Emine GokceDavid C Muddiman
Apr 23, 2013·Methods : a Companion to Methods in Enzymology·Genna L Andrews KingonAdam M Hawkridge
Jun 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shanfa LuVincent L Chiang
Aug 6, 2013·Journal of the American Society for Mass Spectrometry·Shan M RandallDavid C Muddiman

❮ Previous
Next ❯

Citations

Jul 7, 2019·Environmental Science and Pollution Research International·Karina Lima Reis BorgesRicardo Antunes Azevedo
Jan 31, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yan LiuZhiqiang Lv
Jan 28, 2017·Journal of the American Society for Mass Spectrometry·Elizabeth S HechtDavid C Muddiman
Apr 25, 2017·Analytical Chemistry·Qun ZhaoYukui Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.