Apr 23, 2020

Unveiling cells' local environment during cryopreservation by correlative in situ spatial and thermal analyses

BioRxiv : the Preprint Server for Biology
K. QinFrancisco M. Fernandes

Abstract

Cryopreservation is the only fully established procedure to extend the lifespan of living cells and tissues, a key to activities spanning from fundamental biology to clinical practice. Despite its prevalence and impact, central aspects of cryopreservation, such as the cell's physico-chemical environment during freezing, remain elusive. Here we address that question by coupling in situ microscopic directional freezing to visualize cells and their surroundings during freezing with the freezing medium phase diagram. We extract the freezing medium spatial distribution in cryopreservation, providing a tool to describe the cell vicinity at any point during freezing. We show that two major events define the cells' local environment over time: the interaction with the moving ice front and with the vitreous moving front - a term we introduce here. Our correlative strategy may be applied to cells relevant in clinical research and practice, and help designing new cryoprotective media based on local physico-chemical cues.

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Mentioned in this Paper

Plasmodium
CFC1 gene
Genome
Phyllostachys vivax
Plasmodium malariae
Plasmodium vivax
Plasmodium ovale
Malaria
Malaria Vaccines
Genomics

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