Embryo lipoproteins and yolk lipovitellin consumption during embryogenesis in Macrobrachium borellii (Crustacea: Palaemonidae)

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Fernando GarciaHoracio Heras

Abstract

The prawn Macrobrachium borellii has lecithotrophic eggs with highly-abbreviated development. The major yolk component is lipovitellin (LV), a lipoprotein with 30% lipids (by weight). LV consumption during embryogenesis was followed by ELISA and Western blot analysis using an anti-LV polyclonal antibody. No cross-reacting proteins were observed and LV-like lipoproteins were strongly recognized by the antibody in hemolymph (vitellogenin), yolk (LV) and embryos (LVe), as determined by Western Blot analysis. LV decreased significantly along development from 9.4 to 1.1 microg/mg egg. Consumption rate of LV was slow in early embryogenesis, followed by a rapid utilization in late embryonic stages. Significant LVe amounts were still present at hatching. LV apolipoproteins were selectively degraded during embryo development, being the highest molecular weight subunit the most affected. Comparison among in vitro, in vivo and theoretical proteolysis suggested that trypsin may be involved in LV degradation during late embryogenesis. Embryo lipoprotein (HDLe) synthesis was first detected at stage 6. HDLe shared the same density, MW and subunit composition as adult hemolymph HDL(1) and did not cross-react with LV-like lipoproteins. Though e...Continue Reading

References

Feb 2, 2000·The Journal of Experimental Zoology·M R González-BaróR J Pollero
Nov 18, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jongruk AuttaratPrapaporn Utarabhand
Jan 13, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Anna WalkerRichard F Lee
Oct 13, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C F GarciaR Pollero
Apr 24, 2007·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Takuji OkumuraKazutaka Sakiyama

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Citations

Apr 27, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Aldana LainoC Fernando Garcia
Nov 3, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C F GarciaH A Garda
Aug 19, 2015·Fish & Shellfish Immunology·Shicui ZhangPengfei Cui
Jun 19, 2012·Marine Pollution Bulletin·C Fernando García, Horacio Heras
Dec 11, 2019·Environmental Science and Pollution Research International·Gabriela R SilveyraDaniel A Medesani
Apr 7, 2019·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·S RomeroM Cunningham

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