Embryo-specific reduction of ADP-Glc pyrophosphorylase leads to an inhibition of starch synthesis and a delay in oil accumulation in developing seeds of oilseed rape

Plant Physiology
Helene VigeolasPeter Geigenberger

Abstract

In oil-storing Brassica napus (rape) seeds, starch deposition occurs only transiently in the early stages of development, and starch is absent from mature seeds. This work investigates the influence of a reduction of ADP-Glc pyrophosphorylase (AGPase) on storage metabolism in these seeds. To manipulate the activity of AGPase in a seed-specific manner, a cDNA encoding the small subunit of AGPase was expressed in the sense or antisense orientation under the control of an embryo-specific thioesterase promoter. Lines were selected showing an embryo-specific decrease in AGPase due to antisense and cosuppression at different stages of development. At early developmental stages (25 days after flowering), a 50% decrease in AGPase activity was accompanied by similar decreases in starch content and the rate of starch synthesis measured by injecting (14)C-Suc into seeds in planta. In parallel to inhibition of starch synthesis, the level of ADP-Glc decreased, whereas Glc 1-phosphate levels increased, providing biochemical evidence that inhibition of starch synthesis was due to repression of AGPase. At 25 days after flowering, repression of starch synthesis also led to a decrease in the rate of (14)C-Suc degradation and its further metaboli...Continue Reading

References

Oct 1, 1992·Journal of Bacteriology·C Benning, C R Somerville
Jan 5, 2002·Progress in Lipid Research·Stephen Rawsthorne
May 1, 1997·Plant Physiology·S. P. KingR. T. Furbank
Jan 1, 2003·Journal of Experimental Botany·Peter Geigenberger
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

Nov 28, 2008·Journal of Experimental Botany·Asa EkmanSten Stymne
Dec 30, 2015·Plant Physiology and Biochemistry : PPB·Patricia Coello, Eleazar Martínez-Barajas
Jan 17, 2016·Plant Physiology and Biochemistry : PPB·Nosheen ElahiClaudio Stasolla
Aug 25, 2015·Plant Physiology and Biochemistry : PPB·Nosheen ElahiClaudio Stasolla
Aug 17, 2005·The New Phytologist·Ljudmilla BorisjukHardy Rolletschek
May 28, 2010·Biotechnology and Bioengineering·Yantao LiQiang Hu
Oct 1, 2011·The Plant Journal : for Cell and Molecular Biology·Wei HuaHan-Zhong Wang
Jun 16, 2010·The New Phytologist·Vasilios M E AndriotisAlison M Smith
Jun 12, 2013·Progress in Lipid Research·Ljudmilla BorisjukThomas Neuberger
Oct 28, 2014·Frontiers in Plant Science·Volodymyr Radchuk, Ljudmilla Borisjuk
May 5, 2016·The Plant Journal : for Cell and Molecular Biology·Chloé GuerinStéphane Dussert
Oct 6, 2017·Journal of Experimental Botany·Gregory J MacNeillMichael J Emes
May 28, 2013·The Plant Cell·Ljudmilla BorisjukHardy Rolletschek

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