Embryoid body formation of human amniotic fluid stem cells depends on mTOR.

Oncogene
A ValliM Hengstschläger

Abstract

Human amniotic fluid stem cells (hAFSCs) harbor high proliferative capacity and high differentiation potential and do not raise the ethical concerns associated with human embryonic stem cells. The formation of three-dimensional aggregates known as embryoid bodies (EBs) is the principal step in the differentiation of pluripotent embryonic stem cells. Using c-Kit-positive hAFSC lines, we show here that these stem cells harbor the potential to form EBs. As part of the two kinase complexes, mTORC1 and mTORC2, mammalian target of rapamycin (mTOR) is the key component of an important signaling pathway, which is involved in the regulation of cell proliferation, growth, tumor development and differentiation. Blocking intracellular mTOR activity through the inhibitor rapamycin or through specific small interfering RNA approaches revealed hAFSC EB formation to depend on mTORC1 and mTORC2. These findings demonstrate hAFSCs to be a new and powerful biological system to recapitulate the three-dimensional and tissue level contexts of in vivo development and identify the mTOR pathway to be essential for this process.

References

Jul 21, 2001·Stem Cells·M Pesce, H R Schöler
Oct 3, 2001·Nature Biotechnology·C XuM K Carpenter
Jul 2, 2003·Human Reproduction·Andrea-Romana PrusaMarkus Hengstschläger
Aug 6, 2003·Blood·Pieternella S In 't AnkerHumphrey H H Kanhai
Aug 6, 2004·American Journal of Obstetrics and Gynecology·Andrea-Romana PrusaMarkus Hengstschläger
Jan 8, 2005·American Journal of Medical Genetics. Part a·Markus HengstschlägerGerhard Bernaschek
Feb 10, 2006·Cell Research·Cristina BlazquezFrancesco Pezzella
Feb 14, 2006·Cell·Stephan WullschlegerMichael N Hall
Apr 11, 2006·Molecular Cell·Dos D SarbassovDavid M Sabatini
Jan 9, 2007·Nature Biotechnology·Paolo De CoppiAnthony Atala
Jan 11, 2007·Nature Biotechnology·Alan Trounson
Apr 25, 2007·Trends in Molecular Medicine·Stephen G DannGeorge Thomas
May 2, 2007·Human Molecular Genetics·Margit RosnerMarkus Hengstschläger
Jul 5, 2007·Journal of Bioscience and Bioengineering·Hiroshi Kurosawa
Jul 7, 2007·Cancer Cell·David A Guertin, David M Sabatini
Aug 2, 2007·Journal of Molecular Histology·Yash M KolambkarRobert E Guldberg
Aug 8, 2007·Cell Research·Qian Yang, Kun-Liang Guan
Oct 24, 2007·Stem Cell Reviews·Nicol SiegelMarkus Hengstschläger
Nov 21, 2007·Cell Proliferation·L PerinR E De Filippo
Dec 18, 2007·International Journal of Geriatric Psychiatry·C A LynchB A Lawlor
Apr 30, 2008·Methods in Cell Biology·Laura PerinRoger De Filippo
Jul 5, 2008·Mutation Research·Margit RosnerMarkus Hengstschläger
Aug 12, 2008·Cell·In-Hyun ParkGeorge Q Daley
Oct 15, 2008·Nature Clinical Practice. Urology·Tamer Aboushwareb, Anthony Atala
Nov 22, 2008·Cold Spring Harbor Symposia on Quantitative Biology·N Maherali, K Hochedlinger
Feb 18, 2009·Blood·Andrea DitadiIsabelle André-Schmutz
Mar 20, 2009·Reproductive Biomedicine Online·Mara CananziPaolo De Coppi

❮ Previous
Next ❯

Citations

Jun 12, 2010·Nature Protocols·Margit RosnerMarkus Hengstschläger
Nov 2, 2012·The New England Journal of Medicine·Margit Rosner, Markus Hengstschläger
Sep 13, 2011·Stem Cells and Development·Weerapong PrasongcheanPatrizia Ferretti
Dec 20, 2012·Stem Cells and Development·Tatsanee PhermthaiPuttachart Pokathikorn
Jun 15, 2010·Human Molecular Genetics·Nicol SiegelMarkus Hengstschläger
Nov 18, 2011·Human Molecular Genetics·Christiane FuchsMarkus Hengstschläger
Aug 27, 2010·Journal of the Royal Society, Interface·Hassan AbdulrazzakPascale V Guillot
Dec 1, 2012·Stem Cells Translational Medicine·Aleksander SkardalShay Soker
Oct 25, 2012·Stem Cells International·Kerry RennieMahmud Bani-Yaghoub
Jan 11, 2012·Asian Journal of Andrology·Claudia GundackerMarkus Hengstschläger
Jul 14, 2012·Stem Cells International·Anna JezierskiMahmud Bani-Yaghoub
Aug 8, 2012·Stem Cell Reviews and Reports·Andrea PreitschopfMario Mikula
May 17, 2011·Amino Acids·M RosnerM Hengstschläger
Oct 1, 2013·Amino Acids·Margit Rosner, Markus Hengstschläger
Mar 25, 2014·Expert Opinion on Biological Therapy·Anna BajekTomasz Drewa
Nov 6, 2012·Journal of the Mechanical Behavior of Biomedical Materials·Aleksander SkardalShay Soker
Sep 1, 2011·Kidney International Supplements·Irene L NoronhaSergio P Bydlowski
Sep 18, 2012·Progress in Neurobiology·Zhao Zhong ChongKenneth Maiese
Sep 4, 2012·Mutation Research·Nina KramerHelmut Dolznig
Aug 4, 2010·Stem Cells·Guangyu AnCarol F Webb
Apr 27, 2013·Cell Biology International·Liyang GaoNorshariza Nordin
Sep 30, 2010·Journal of Cellular and Molecular Medicine·Elisabetta CenniNicola Baldini
Sep 29, 2015·Research in Veterinary Science·Hoo Mun-FunNorshariza Nordin
Mar 19, 2015·Journal of Cellular and Molecular Medicine·Rita RomaniFrancesca Fallarino
Mar 27, 2015·Molecular BioSystems·Rita RomaniFrancesca Magherini
Sep 11, 2012·Organogenesis·Margit RosnerMarkus Hengstschläger
Oct 6, 2012·Organogenesis·Mara Cananzi, Paolo De Coppi
Jan 23, 2013·Trends in Molecular Medicine·Margit Rosner, Markus Hengstschläger
Jul 30, 2014·Stem Cells and Development·Mariano Di TrapaniMauro Krampera
Apr 26, 2016·International Journal of Molecular Sciences·Ivana AntonucciLiborio Stuppia
Jun 22, 2012·Stem Cells International·Margit RosnerMarkus Hengstschläger
Apr 3, 2014·Stem Cells Translational Medicine·Margit RosnerMarkus Hengstschläger
May 10, 2019·International Journal of Molecular Sciences·Melissa RodriguesLiborio Stuppia
Nov 17, 2016·Stem Cell Research & Therapy·Areechun SotthibundhuSanbing Shen
Jan 15, 2021·International Journal of Molecular Sciences·Margit RosnerMarkus Hengstschläger
Aug 13, 2021·Stem Cell Reviews and Reports·Margit RosnerMarkus Hengstschläger

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.