Embryonal carcinoma cell adhesion: the role of surface galactosyltransferase and its 90K lactosaminoglycan substrate

Developmental Biology
B D Shur

Abstract

Embryonal carcinoma (EC) cells possess a complex cell surface glycoconjugate called lactosaminoglycan, whose core structure is composed of repeating N-acetyllactosamine (Gal leads to GlcNAc) disaccharides. Recent studies suggest that the cell surface receptor for lactosaminoglycan is galactosyltransferase, which binds terminal GlcNAc residues on various side chains, thus anchoring the glycoconjugate to the cell surface (Shur, B. D. (1982). J. Biol. Chem. 257, 6871-6878.). The results described in this paper suggest that multivalent lactosaminoglycans mediate EC cell adhesions by binding to their surface galactosyltransferase receptors. In the presence of UDPgalactose, but not other sugar nucleotides, EC cell adhesion is reduced and preformed cell adhesions are dissociated. UDPgalactose interferes with EC cell adhesion by forcing the galactosyltransferase reaction to completion, thus dissociating the enzyme from its galactosylated substrate (i.e., lactosaminoglycan), and thereby dissociating EC cells from one another. Lactosaminoglycans purified from EC cell cultures rapidly agglutinate EC cells, and EC cells preferentially adhere to substrates irreversibly derivatized with protein- and lipid-free lactosaminoglycan side chains. ...Continue Reading

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Citations

Mar 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S C EvansB D Shur
Mar 1, 1991·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·P J McCormick
Jun 1, 1985·Developmental Biology·M De FeliciG Cossu
Mar 1, 1986·Developmental Biology·B A FendersonS Hakomori
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Apr 1, 1990·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·B A FendersonS Hakomori
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Nov 1, 1988·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·H J Hathaway, B D Shur
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