Embryonic and postnatal tendon cells respond differently to interleukin-1β

Annals of the New York Academy of Sciences
Jiewen LiCatherine K Kuo

Abstract

Adult tendons heal as scar tissue, whereas embryonic tendons heal scarlessly via unknown mechanisms. Scarred tendon healing results from inflammation-driven imbalances in anabolic and catabolic functions. To test scarless versus scarring age tendon cell responses to inflammatory conditions, we treated embryonic and postnatal tendon cells with interleukin (IL)-1β and characterized expression of collagens, matrix metalloproteinases (MMPs), inflammatory mediators, and phosphorylation of signaling molecules. At baseline, postnatal cells expressed significantly higher levels of inflammatory mediators. When treated with IL-1β, both postnatal and embryonic cells upregulated inflammatory mediators and MMPs. Notably, postnatal cells secreted inflammatory factors up to 12.5 times the concentration in embryonic cultures. IL-1β activated NF-κB p65 and p38 mitogen-activated protein kinase (MAPK) pathways in both cell types, but phosphorylated p38 MAPK levels were two times higher in postnatal than embryonic cells. Our results suggest that scarred healing tendon cells respond to proinflammatory cytokines by promoting an imbalance in anabolic and catabolic functions, and that the heightened response involves p38 MAPK signaling activity. In co...Continue Reading

References

Jan 1, 1991·Annual Review of Immunology·M J Bosma, A M Carroll
Apr 1, 1991·The Journal of Surgical Research·M T LongakerN S Adzick
Nov 1, 1995·Plastic and Reconstructive Surgery·H P LorenzN S Adzick
Jun 1, 1995·The Journal of Hand Surgery : Journal of the British Society for Surgery of the Hand·M M al-QattanK Y Lin
Mar 1, 1995·Journal of Pediatric Surgery·M MeuliN S Adzick
Jan 1, 1994·Annals of Surgery·M T LongakerN S Adzick
Jul 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J E SimsK Shanebeck
Feb 12, 1996·FEBS Letters·A J FosangP J Neame
Feb 21, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Joanne ArchambaultAlbert J Banes
Dec 6, 2002·Nature·UNKNOWN Mouse Genome Sequencing ConsortiumEric S Lander
Feb 6, 2003·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M TsuzakiA J Banes
Sep 26, 2003·The Journal of Hand Surgery·Wataru OshiroPaul R Manske
Dec 3, 2003·Annals of Biomedical Engineering·Pedro K BeredjiklianLouis J Soslowsky
Jul 27, 2004·Clinical Journal of Sport Medicine : Official Journal of the Canadian Academy of Sport Medicine·Feridun CilliJames H-C Wang
Aug 7, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Anthony N CorpsGraham P Riley
Aug 28, 2004·The American Journal of Pathology·Traci A WilgusTatiana M Oberyszyn
Nov 8, 2005·Journal of Biomechanics·Tony W LinLouis J Soslowsky
Apr 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuko IshidaNaofumi Mukaida
Jun 15, 2006·Plastic and Reconstructive Surgery·Amy S ColwellH Peter Lorenz
Sep 1, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Michele FavataLouis J Soslowsky
Sep 28, 2006·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Hiroshi KoshimaNaoki Ishiguro
Oct 24, 2007·The Journal of Hand Surgery, European Volume·M BerglundM Wiig
Nov 29, 2007·The European Journal of Health Economics : HEPAC : Health Economics in Prevention and Care·Johan JarlRobin Room
Apr 22, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Catherine K KuoRocky S Tuan
May 13, 2008·Clinical Orthopaedics and Related Research·Hui B SunEvan L Flatow
Sep 2, 2008·Tissue Engineering. Part a·Catherine K Kuo, Rocky S Tuan
Feb 25, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Spences S WatsonRonen Schweitzer
Oct 6, 2009·Journal of Shoulder and Elbow Surgery·Asheesh BediScott A Rodeo
Feb 4, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Thilo JohnGundula Schulze-Tanzil
Feb 8, 2011·Biochimica Et Biophysica Acta·Jürgen SchellerStefan Rose-John
Feb 26, 2011·Journal of Applied Physiology·Mette Bisgaard AndersenHenning Langberg
May 5, 2011·Matrix Biology : Journal of the International Society for Matrix Biology·Barbara Gawronska-Kozak
Feb 18, 2012·Rheumatology·Kirsten LegerlotzGraham P Riley
Sep 18, 2012·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·P W AckermannG A Nader
Apr 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Joseph E MarturanoCatherine K Kuo

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Citations

Dec 16, 2019·British Medical Bulletin·Emanuele ChisariNicola Maffulli
Feb 26, 2020·BMC Biomedical Engineering·Sophia K Theodossiou, Nathan R Schiele
Jan 28, 2021·Stem Cell Research & Therapy·Sophia K TheodossiouNathan R Schiele
Mar 9, 2021·Frontiers in Cell and Developmental Biology·Zizhan HuangWeiliang Shen

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