Embryonic and uterine expression patterns of peptidylglycine alpha-amidating monooxygenase transcripts suggest a widespread role for amidated peptides in development

Developmental Biology
J ZhangJ E Pintar

Abstract

Posttranslational processing of peptide precursors frequently includes COOH-terminal amidation by the bifunctional enzyme peptidylglycine alpha-amidating monooxygenase (PAM). We examined the ontogeny of PAM gene expression using in situ hybridization and detected expression in the cardiogenic region beginning at embryonic day 9 (e9) and in decidualizing uterine endometrium and myometrial smooth muscle at even earlier postimplantation stages. PAM expression in the CNS at e10 was highest in the dorsal spinal cord and floor plate and exhibited complex patterning in several CNS regions, including the ventricular zone, over the next several days with PAM expression first detected in neurons at e13. High levels of PAM expression characterized several nonneural cell populations as well, including limb mesoderm and the mesenchyme immediately adjacent to nasal, maxillary, palatal, and dental epithelia during tissue fusion and remodeling. Since alternative splicing generates PAM transcripts encoding proproteins that are differentially localized and processed, we used probes that distinguish major subsets of PAM transcripts to determine that transcripts encoding integral membrane PAM isoforms predominate in most, if not all, PAM-expressin...Continue Reading

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Citations

Apr 10, 2009·BMC Genomics·Yao Fei HuMaya Sieber-Blum
Dec 19, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·María J Sánchez de Miguel, María A Burrell
Oct 5, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Oihana GarmendiaAna C Villaro
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Sep 1, 2004·The Journal of Biological Chemistry·Xiaonan XinBetty A Eipper
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Mar 29, 2001·Molecular and Cellular Neurosciences·Z G RenM Sieber-Blum
Oct 11, 2003·Molecular and Cellular Neurosciences·Y H YounM Sieber-Blum

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