Embryonic chick retinal ganglion cells identified "in vitro". Their survival is dependent on a factor from the optic tectum

Experimental Brain Research
V Nurcombe, M R Bennett

Abstract

When HRP is injected into the optic tecta of embryonic or newly hatched chicks, the ganglion cells in the contralateral retina can be successfully dissociated into culture and identified at any time by appropriate histochemical staining. Histological examination of whole mounts of retinae both ipsilateral and contralateral to an injection site indicated that no HRP diffused out of an injected tectum, and that the only reaction product that could be visualized was restricted to the ganglion cell layer of the contralateral eye. Because retinal ganglion cells are the only retinal neurons to project to the optic tectum, the intraxonal retrograde transport of HRP to these cells allows their unequivocal identification from amongst the heterogeneous population of retinal neurons present after dispersal into single cells in monolayer culture. The presence of HRP in the cell bodies did not appear to impair their ability to survive, grow or express neurites. Counts of labeled cells from progressively aged birds confirmed that the peak number of generated ganglion cells occurs on embryonic day 10,and that is a 40% decline in the number these neurons over the following 3 days. However, when labelled ganglion cells from 10 day embryos were ...Continue Reading

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