Embryonic chicken trachea as a new in vitro model for the investigation of mucociliary particle clearance in the airways.

AAPS PharmSciTech
Andreas HenningC-M Lehr

Abstract

Mucociliary clearance (MC) is an important defense mechanism of the respiratory system to eliminate inhaled and possibly noxious particles from the lung. Although the principal mechanics of MC seem to be relatively clear there are still open questions regarding the long-term clearance of particles. Therefore, we have developed a new set-up based on embryonic chicken trachea (ECT) to investigate mucociliary particle clearance in more detail. ECT was placed in an incubation chamber after carbon particles were applied and tracked using optical microscopy. The aim of the study was to validate this model by investigating the impact of temperature, humidity and drugs on particle transport rates. Particles were transported reproducibly along the trachea and clearance velocity (2.39 +/- 0.25) mm/min was found to be in accordance to data reported in literature. Variation in temperature resulted in significantly reduced MC: (0.40 +/- 0.12) mm/min (20 degrees C); (0.42 +/- 0.10) mm/min (45 degrees C). Decreasing humidity (99-60%) had no significant effect on MC, whereas reduction to 20% humidity showed a significant influence on particle clearance. The use of different cilio- and muco-active drugs (propranolol, terbutalin, N-acetylcystein...Continue Reading

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Citations

May 27, 2010·Journal of Aerosol Medicine and Pulmonary Drug Delivery·Andreas HenningClaus-Michael Lehr
Apr 15, 2014·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Pall Thor IngvarssonCamilla Foged
Jun 30, 2009·Inhalation Toxicology·Michael BurClaus-Michael Lehr
May 24, 2011·Expert Opinion on Drug Delivery·José das NevesBruno Sarmento
Dec 3, 2014·Advanced Drug Delivery Reviews·Marius HittingerClaus-Michael Lehr
Jan 10, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Julian KirchClaus-Michael Lehr
Jan 2, 2013·Journal of Biomechanics·J HussongJ Westerweel
May 11, 2018·The Korean Journal of Parasitology·Maha M A BasyoniDalia Ibrahim Ismail
Jun 19, 2021·Biotechnology and Bioengineering·Lucia PedersoliDaniela Peneda Pacheco

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