Embryonic disc formation following post-hatching bovine embryo development in vitro.

Reproduction : the Official Journal of the Society for the Study of Fertility
Priscila Ramos-IbeasPablo Bermejo-Álvarez

Abstract

Failures during conceptus elongation are a major cause of pregnancy losses in ungulates, exerting a relevant economic impact on farming. The developmental events occurring during this period are poorly understood, mainly because this process cannot be recapitulated in vitro. Previous studies have established an in vitro post-hatching development (PHD) system that supports bovine embryo development beyond the blastocyst stage, based on agarose gel tunnels and serum- and glucose-enriched medium. Unfortunately, under this system embryonic disc formation is not achieved and embryos show notorious signs of apoptosis and necrosis. The objective of this study has been to develop an in vitro system able to support embryonic disc formation. We first compared post-hatching development inside agarose tunnels or free-floating over an agarose-coated dish in serum- and glucose-enriched medium (PHD medium). Culture inside agarose tunnels shaped embryo morphology by physical constriction, but it restricted embryo growth and did not provide any significant advantage in terms of development of hypoblast and epiblast lineages. In contrast to PHD medium, a chemically defined and enriched medium (N2B27) supported complete hypoblast migration and ep...Continue Reading

Citations

Dec 29, 2020·Theriogenology·Ekaterina Isaac, Peter L Pfeffer
Feb 17, 2021·Biology of Reproduction·Constantine A SimintirasPatrick Lonergan
May 1, 2021·Journal of Developmental Biology·Claudia SpringerKilian Simmet
Jul 3, 2021·Frontiers in Veterinary Science·Alba Pérez-GómezPriscila Ramos-Ibeas

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Methods Mentioned

BETA
FCS
PCR
electron microscopy
transmission electron microscopy
confocal microscopy

Software Mentioned

Fiji
GraphPad Prism

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis