Embryonic exposure to the fungicide vinclozolin causes virilization of females and alteration of progesterone receptor expression in vivo: an experimental study in mice

Environmental Health : a Global Access Science Source
Jill BuckleyLaurence S Baskin

Abstract

Vinclozolin is a fungicide that has been reported to have anti-androgenic effects in rats. We have found that in utero exposure to natural or synthetic progesterones can induce hypospadias in mice, and that the synthetic progesterone medroxyprogesterone acetate (MPA) feminizes male and virilizes female genital tubercles. In the current work, we selected a relatively low dose of vinclozolin to examine its in utero effects on the development of the genital tubercle, both at the morphological and molecular levels. We gave pregnant dams vinclozolin by oral gavage from gestational days 13 through 17. We assessed the fetal genital tubercles from exposed fetuses at E19 to determine location of the urethral opening. After determination of gonadal sex, either genital tubercles were harvested for mRNA quantitation, or urethras were injected with a plastic resin for casting. We analyzed quantified mRNA levels between treated and untreated animals for mRNA levels of estrogen receptors alpha and beta, progesterone receptor, and androgen receptor using nonparametric tests or ANOVA. To determine effects on urethral length (males have long urethras compared to females), we measured the lengths of the casts and performed ANOVA analysis on these...Continue Reading

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Citations

Feb 19, 2013·Journal of Mammary Gland Biology and Neoplasia·Madisa B Macon, Suzanne E Fenton
Aug 8, 2008·Journal of Toxicology and Environmental Health. Part a·Xing LiuGuang-Hui Wei
Mar 17, 2015·Epigenetics : Official Journal of the DNA Methylation Society·Josep C Jiménez-ChillarónPaulo J Oliveira
Apr 9, 2015·Nature Reviews. Urology·Gerald R CunhaLaurence S Baskin
Jul 26, 2016·Frontiers in Neuroscience·Adel DerghalLourdes Mounien
Jul 5, 2015·Theriogenology·Malgorzata KnetMalgorzata Duda
Aug 6, 2014·Reproductive Toxicology·Gwendolyn OsborneMegan Schwarzman
Oct 25, 2008·Journal of Pediatric Urology·Laurence S Baskin
Nov 11, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Christelle Stouder, Ariane Paoloni-Giacobino
Jul 4, 2006·The Journal of Urology·Emily WillinghamLaurence S Baskin
May 8, 2007·Nature Clinical Practice. Urology·Emily Willingham, Laurence S Baskin
Apr 19, 2018·Biology of Reproduction·Ciro M AmatoKrista A McCoy
Dec 17, 2020·Environmental Epigenetics·Jake D Lehle, John R McCarrey
Mar 21, 2021·Developmental Biology·Nelson R Cabej
May 18, 2021·Pesticide Biochemistry and Physiology·Changwon YangGwonhwa Song

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Methods Mentioned

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cesarean section

Software Mentioned

Adobe Photoshop

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