Embryonic GABAergic spinal commissural neurons project rostrally to mesencephalic targets

The Journal of Comparative Neurology
T S TranP E Phelps

Abstract

Although spinal commissural neurons serve as a model system for studying the mechanisms that underlie axonal pathfinding during development, little is known about their synaptic targets. Previously we identified a group of ventromedially located commissural neurons in rat spinal cord that are gamma-aminobutyric acid (GABA)-ergic and express L1 CAM on their axons. In this study, serial sagittal sections of embryos (E12-15) were processed for glutamic acid decarboxylase (GAD)-65 and L1 immunocytochemistry and showed labeled commissural axons coursing rostrally within the ventral marginal zone. Both GAD65- and L1-positive axons extended rostrally out of the spinal cord into the central part of the medulla and then into the midbrain. GAD65-positive axons branched and ended abruptly within the lateral midbrain. To determine the targets of these ventral commissural neurons, embryos (E13-15) were injected with DiI into the ventromedial spinal cord. At all three ages, DiI-labeled axons projected rostrally in the contralateral ventral marginal zone, turned into the central medulla, and then traveled to the midbrain. DiI-labeled axons appeared to terminate in the lateral midbrain by branching into small, punctate structures. In reciproca...Continue Reading

References

Dec 1, 1975·The Journal of Comparative Neurology·B J McLaughlinJ Y Wu
Nov 22, 1992·The Journal of Comparative Neurology·I Silos-Santiago, W D Snider
Apr 23, 1990·Brain Research·A J Todd, V Lochhead
Sep 8, 1988·The Journal of Comparative Neurology·R W OppenheimH Yaginuma
Sep 1, 1989·Trends in Neurosciences·M G Honig, R I Hume
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C Lagenaur, V Lemmon
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W McLean, P K Nakane
Mar 10, 1982·The Journal of Comparative Neurology·J A Holley
Dec 1, 1995·Brain : a Journal of Neurology·C K Thomas, G Westling
Aug 10, 2000·The Journal of Comparative Neurology·Z KatarovaG Szabó
May 29, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Z KaprielianR Imondi
Jan 2, 2003·The Journal of Comparative Neurology·Tracy S TranPatricia E Phelps

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Citations

May 19, 2009·Developmental Neurobiology·Wendy RockhillMartha M Bosma
Jan 27, 2015·Wiley Interdisciplinary Reviews. Developmental Biology·Edward Martinez, Tracy S Tran
Mar 13, 2014·Neuroscience Letters·Hidemichi KinChitoshi Takayama
Mar 6, 2009·Developmental Neurobiology·Sean M HughesMartha M Bosma
Oct 12, 2007·Physiological Reviews·Yehezkel Ben-AriRustem Khazipov

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