Embryonic gene expression of Coregonus palaea (whitefish) under pathogen stress as analyzed by high-throughput RNA-sequencing

Fish & Shellfish Immunology
Laetitia G E WilkinsLuca Fumagalli

Abstract

Most fishes produce free-living embryos that are exposed to environmental stressors immediately following fertilization, including pathogenic microorganisms. Initial immune protection of embryos involves the chorion, as a protective barrier, and maternally-allocated antimicrobial compounds. At later developmental stages, host-genetic effects influence susceptibility and tolerance, suggesting a direct interaction between embryo genes and pathogens. So far, only a few host genes could be identified that correlate with embryonic survival under pathogen stress in salmonids. Here, we utilized high-throughput RNA-sequencing in order to describe the transcriptional response of a non-model fish, the Alpine whitefish Coregonus palaea, to infection, both in terms of host genes that are likely manipulated by the pathogen, and those involved in an early putative immune response. Embryos were produced in vitro, raised individually, and exposed at the late-eyed stage to a virulent strain of the opportunistic fish pathogen Pseudomonas fluorescens. The pseudomonad increased embryonic mortality and affected gene expression substantially. For example, essential, upregulated metabolic pathways in embryos under pathogen stress included ion binding...Continue Reading

References

Oct 31, 1998·Current Opinion in Genetics & Development·M H Sieweke, T Graf
Dec 10, 1998·Nucleic Acids Research·H OgataM Kanehisa
May 14, 1999·Trends in Ecology & Evolution·A A Hoffmann, J Merilä
Sep 29, 2004·Fish & Shellfish Immunology·Uwe FischerMitsuru Ototake
May 27, 2005·Fish & Shellfish Immunology·B MagnadottirR A Dalmo
Jun 15, 2005·Cellular Microbiology·Hao Lu, Margaret Clarke
Feb 14, 2006·Cellular Microbiology·Patrick FarbrotherLudwig Eichinger
Oct 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hatem E SabaawyDennis D Hickstein
Jun 21, 2007·Diseases of Aquatic Organisms·Oivind Bergh
Mar 20, 2008·Genome Research·Daniel R Zerbino, Ewan Birney
May 1, 2008·Nucleic Acids Research·Stefan GötzAna Conesa
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Jul 30, 2009·Progress in Lipid Research·Ynske P M van der Meer-JanssenJ Bernd Helms
Jan 19, 2010·Bioinformatics·Heng Li, Richard Durbin
Jan 30, 2010·Bioinformatics·Aaron R Quinlan, Ira M Hall
Jul 31, 2010·Nucleic Acids Research·Sudeep Srivastava, Liang Chen
Sep 8, 2010·Fish & Shellfish Immunology·Anita OrdasAnnemarie H Meijer
Sep 14, 2010·Current Protocols in Bioinformatics·Daniel R Zerbino
Nov 3, 2010·Molecular Ecology·Alain JacobClaus Wedekind
Jul 20, 2011·Fish & Shellfish Immunology·Martha Reyes-BecerrilMaría Ángeles Esteban

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Citations

Jun 12, 2020·Journal of Fish Biology·Benjamin Geffroy, Claus Wedekind
Aug 11, 2016·Molecular Ecology·Laetitia G E WilkinsClaus Wedekind
Feb 26, 2019·Frontiers in Immunology·Shun MaekawaShih-Chu Chen

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