Embryonic POU5F1 is Required for Expanded Bovine Blastocyst Formation

Scientific Reports
Bradford W DaigneaultPablo J Ross

Abstract

POU5F1 is a transcription factor and master regulator of cell pluripotency with indispensable roles in early embryo development and cell lineage specification. The role of embryonic POU5F1 in blastocyst formation and cell lineage specification differs between mammalian species but remains completely unknown in cattle. The CRISPR/Cas9 system was utilized for targeted disruption of the POU5F1 gene by direct injection into zygotes. Disruption of the bovine POU5F1 locus prevented blastocyst formation and was associated with embryonic arrest at the morula stage. POU5F1 knockout morulas developed at a similar rate as control embryos and presented a similar number of blastomeres by day 5 of development. Initiation of SOX2 expression by day 5 of development was not affected by lack of POU5F1. On the other hand, CDX2 expression was aberrant in embryos lacking POU5F1. Notably, the phenotype observed in bovine POU5F1 knockout embryos reveals conserved functions associated with loss of human embryonic POU5F1 that differ from Pou5f1- null mice. The similarity observed in transcriptional regulation of early embryo development between cattle and humans combined with highly efficient gene editing techniques make the bovine a valuable model for...Continue Reading

References

Apr 20, 1999·Biology of Reproduction·M J van EijkF Gandolfi
Nov 25, 2000·Biology of Reproduction·N KirchhofH Niemann
Apr 20, 2001·Mammalian Genome : Official Journal of the International Mammalian Genome Society·V NordhoffH R Schöler
Dec 10, 2002·Reproductive Biomedicine Online·Yves J R Ménézo, François Hérubel
Jul 2, 2004·Biology of Reproduction·Satoshi KurosakaK John McLaughlin
Nov 6, 2007·Development·Jens-Erik Dietrich, Takashi Hiiragi
Mar 12, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Ewart W KuijkBernard A J Roelen
Mar 26, 2010·Methods in Molecular Biology·Pablo J Ross, Jose B Cibelli
Feb 15, 2011·Developmental Cell·Debra K BergPeter L Pfeffer
Mar 25, 2011·Nature·Janet Rossant
Nov 7, 2012·BMC Developmental Biology·Manabu OzawaPeter J Hansen
Jul 10, 2013·Molecular Reproduction and Development·Andrew T Schiffmacher, Carol L Keefer
Oct 23, 2013·Biochimica Et Biophysica Acta·Stepan JerabekVlad Cojocaru
Jan 7, 2014·Biology of Reproduction·Marcelo D Goissis, Jose B Cibelli
Sep 25, 2014·Molecular Reproduction and Development·Marcelo D Goissis, Jose B Cibelli
Oct 11, 2014·Nucleic Acids Research·Eva K BrinkmanBas van Steensel
Nov 20, 2014·Cell Regeneration·Guangming Wu, Hans R Schöler
Aug 22, 2015·Development·Paul BlakeleyKathy K Niakan
Oct 22, 2016·Journal of Visualized Experiments : JoVE·Yanina S BogliottiPablo J Ross
Sep 28, 2017·Nature·Norah M E FogartyKathy K Niakan
Feb 28, 2018·Proceedings of the National Academy of Sciences of the United States of America·Kilian SimmetEckhard Wolf

❮ Previous
Next ❯

Citations

Oct 3, 2019·Zygote : the Biology of Gametes and Early Embryos·A E VelásquezL L Rodríguez-Alvarez
Dec 4, 2019·Nature Cell Biology·Rebecca A Lea, Kathy K Niakan
Oct 1, 2020·Scientific Reports·Joseph R OwenAlison L Van Eenennaam
Oct 7, 2020·Annual Review of Cell and Developmental Biology·Claudia GerriKathy K Niakan
Nov 3, 2020·Frontiers in Genetics·Luiz Sergio Almeida CamargoPablo Juan Ross
Oct 1, 2020·Biology of Reproduction·Bradford W Daigneault
Feb 24, 2020·Theriogenology·Ramiro Alberio
Mar 6, 2021·Journal of Equine Veterinary Science·Erin HiseyStuart A Meyers
Mar 9, 2021·Frontiers in Cell and Developmental Biology·Elisa Mariano PioltineMarcelo Fábio Gouveia Nogueira
Jun 22, 2020·Journal of Equine Veterinary Science·Sergio Navarro-SernaPablo Juan Ross
Mar 31, 2021·Journal of Equine Veterinary Science·Erin A HiseyStuart Meyers
Apr 22, 2021·American Journal of Physiology. Cell Physiology·Peter Z Schall, Keith E Latham
Jul 3, 2021·Frontiers in Veterinary Science·Alba Pérez-GómezPriscila Ramos-Ibeas

❮ Previous
Next ❯

Methods Mentioned

BETA
targeted knockout
targeted
genotyping
PCR
electrophoresis

Software Mentioned

TIDE
Axiocam
TIDE Analysis
AddGene designer
SnapGene
ImageJ

Related Concepts

Related Feeds

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

CRISPR for Genome Editing

Genome editing technologies enable the editing of genes to create or correct mutations. Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). Here is the latest research on the use of CRISPR-Cas system in gene editing.

CRISPR Ribonucleases Deactivation

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on mechanisms that underlie deactivation of CRISPR ribonucleases. Here is the latest research.