Embryonic precursor cells that express Trk receptors: induction of different cell fates by NGF, BDNF, NT-3, and CNTF

Experimental Neurology
M B LachyankarA H Ross

Abstract

Epidermal growth factor (EGF)-treated neurosphere cultures from embryonal striatum contain multipotential cells capable of neuronal, astrocytic, and oligodendroglial differentiation. In this study, we tested whether these neural precursor cells differentiate in the presence of neurotrophic factors. We first assayed neurosphere cells for expression of neurotrophin receptors. TrkA, TrkB, TrkC, and gp75 were detected by immunofluorescence microscopy in 60-80% of cells. In addition, the ciliary neurotrophic factor receptor alpha was expressed in 50-60% of cells. In the presence of the mitogen, EGF, treatment of stem cells with neurotrophic factors had no apparent effect. Removal of EGF from cells resulted in cessation of cell proliferation and pronounced astrocytic (glial fibrillary acidic protein+) differentiation. Neuronal (neurofilament+) and oligodendroglial (galactocerebroside+) cells appeared in cultures treated with neurotrophic factors. Nerve growth factor (NGF) resulted in bipolar neuronal cells, and brain-derived neurotrophic factor led to multipolar neuronal cells. Treatment with neurotrophin-3 or ciliary neurotrophic factor resulted in bipolar neuronal cells and oligodendrocytes. Neuronal differentiation in the presence...Continue Reading

References

Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·L A Greene, A S Tischler
May 1, 1994·Trends in Neurosciences·R M LindsayP S DiStefano
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·B Kirschenbaum, S A Goldman
May 1, 1994·Molecular Biology of the Cell·D O ClaryL F Reichardt
Mar 1, 1996·The Journal of Cell Biology·A H RossD E Wolf
Dec 1, 1995·Current Opinion in Cell Biology·M C Birling, J Price
Feb 1, 1996·Neuron·W D Snider, D E Wright

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Citations

May 2, 2008·Stem Cells and Development·J MokrýD English
Sep 20, 2008·Tissue Engineering. Part a·Stephanie M Willerth, Shelly E Sakiyama-Elbert
Jul 11, 2006·Neuro-Signals·Klaus HeeseNoriko Inoue
May 23, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Ayelet Cohen-YeshurunRonen R Leker
Jun 23, 2007·Stem Cells·Stephanie M WillerthShelly E Sakiyama-Elbert
Feb 19, 2008·The European Journal of Neuroscience·Ryo SasakiHideki Ogiuchi
Sep 22, 2006·The European Journal of Neuroscience·Topi A TervonenMaija Castrén
Oct 10, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Sophia L B OliveiraHenning Ulrich
Oct 19, 2004·Neuroscience Letters·Dong Hoon ShinSa Sun Cho
Feb 19, 2003·Biochemical and Biophysical Research Communications·Sanae HosomiMasaya Tohyama
Aug 4, 2015·Neurochemical Research·Sophia L B OliveiraHenning Ulrich
Jun 12, 2002·Molecular and Cellular Neurosciences·Li LiAlonzo H Ross
Feb 13, 2001·Experimental Neurology·D J LieblL F Parada
Jul 23, 1999·Experimental Neurology·M K CarpenterL U Wahlberg
Jun 9, 2005·Progress in Retinal and Eye Research·Ani V DasIqbal Ahmad
Feb 19, 2000·Journal of Neuroscience Research·G J BrookerP F Bartlett
Oct 19, 1999·The Journal of Comparative Neurology·S L CochranE W Rubel
Sep 3, 2004·Behavioural Pharmacology·I BranchiE Alleva
Feb 19, 2000·Journal of Neuroscience Research·R LiD W Sah

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