Embryonic stem cells do not stiffen on rigid substrates.

Biophysical Journal
Yeh-Chuin PohNing Wang

Abstract

It has been previously established that living cells, including mesenchymal stem cells, stiffen in response to elevation of substrate stiffness. This stiffening is largely attributed to the elevation of the tractions at the cell base that is associated with increases in cell spreading on more-rigid substrates. We show here, surprisingly, that mouse embryonic stem cells (ESCs) do not stiffen when substrate stiffness increases. As shown recently, these cells do not increase spreading on more-rigid substrates either. However, these ESCs do increase their basal tractions as substrate stiffness increases. We conclude that these ESCs exhibit mechanical behaviors distinct from those of mesenchymal stem cells and of terminally differentiated cells, and decouple its apical cell stiffness from its basal tractional stresses during the substrate rigidity response.

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Citations

Jul 4, 2012·Nature Materials·Jing LiuBo Huang
Jul 14, 2011·Physical Biology·Michelangelo von Dassow, Lance A Davidson
Jun 1, 2012·Stem Cells and Development·Anna JagielskaRobin J M Franklin
Mar 12, 2013·Tissue Engineering. Part B, Reviews·Jonathan K EarlsKaiming Ye
May 7, 2013·Annual Review of Biomedical Engineering·Kristian FranzeJochen Guck
Nov 2, 2012·Stem Cell Research & Therapy·Evelyn K F Yim, Michael P Sheetz
Mar 12, 2011·Regenerative Medicine·Dong LiFei Wang
May 31, 2014·Nature Communications·Yeh-Chuin PohNing Wang
Dec 16, 2011·Regenerative Medicine·Dekel DadoAssaf Zemel
May 23, 2014·Nature Materials·Ning Wang
Jul 15, 2015·Acta Biomaterialia·Shahzad AliFarlan S Veraitch
Nov 17, 2010·Biochemical and Biophysical Research Communications·Kazushi TamuraKazushige Kawabata
Nov 1, 2011·Biochemical and Biophysical Research Communications·Yuhei UdaNing Wang
Apr 28, 2017·Annals of Biomedical Engineering·Manisha K ShahEric M Darling
Nov 26, 2015·Advanced Healthcare Materials·Yanfen Li, Kristopher A Kilian
Nov 22, 2019·Physiological Reviews·Paul A JanmeyCynthia A Reinhart-King
Aug 12, 2019·ACS Biomaterials Science & Engineering·Shumin XiaPakorn Kanchanawong

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