EMC6/TMEM93 suppresses glioblastoma proliferation by modulating autophagy

Cell Death & Disease
X ShenY Bai

Abstract

EMC6 (endoplasmic reticulum membrane protein complex subunit 6), also known as transmembrane protein 93, is a novel positive autophagy regulator. In this report, we evaluated the anti-tumor activity of EMC6 in glioblastoma cells in vitro and in vivo. Our data show that overexpression of EMC6 in three glioblastoma cell lines (SHG44, U87 and U251) suppresses tumor cell growth by activating autophagy, but fails to induce cell apoptosis. EMC6-mediated autophagy was associated with inactivation of the PIK3CA/AKT/mTOR signaling pathway. Accordingly, EMC6 knockdown in glioblastoma cells had the opposite effect; it promoted cell growth. Overexpression of EMC6 also sensitized glioblastoma cells to the chemotherapy drug, temozolomide, to further suppress tumor growth. Our data indicate that EMC6-induced autophagy may play a positive role in suppressing the development of glioblastoma.

References

Jan 15, 1983·International Journal of Cancer. Journal International Du Cancer·J GerdesH Stein
Jan 19, 1999·Journal of Neuro-oncology·T S SurawiczH R Menck
Nov 2, 2001·Trends in Molecular Medicine·P K Vogt
Dec 6, 2001·Cell·S MisraJ H Hurley
Jul 3, 2002·Nature Reviews. Cancer·Igor Vivanco, Charles L Sawyers
Nov 29, 2002·Eukaryotic Cell·Fulvio Reggiori, Daniel J Klionsky
Jan 10, 2004·Cell Death and Differentiation·T KanzawaS Kondo
Apr 18, 2009·Biochimica Et Biophysica Acta·Eugenia MorselliGuido Kroemer
May 12, 2009·Acta Biochimica Et Biophysica Sinica·Wenzhuo ZhuangZhongqin Liang
Jun 16, 2009·Cell·Robin MathewEileen White
Dec 26, 2009·Current Opinion in Cell Biology·Zhifen Yang, Daniel J Klionsky
May 7, 2010·CA: a Cancer Journal for Clinicians·Erwin G Van MeirJeffrey J Olson
Aug 18, 2010·The Journal of Cell Biology·Kohichi MatsunagaTamotsu Yoshimori
Sep 3, 2010·Nature Cell Biology·Noboru Mizushima, Beth Levine
Sep 25, 2010·Journal of Clinical Neuroscience : Official Journal of the Neurosurgical Society of Australasia·Xin HuangYi-Cheng Lu
Dec 4, 2010·Science·Joshua D Rabinowitz, Eileen White
May 20, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Hui-Wen ChiuYing-Jan Wang
Dec 14, 2011·Brain Pathology·Niroop KazaKevin A Roth
Apr 17, 2012·Cell·Mathieu Laplante, David M Sabatini
Jan 1, 2012·Cells·Francesca ArediaA Ivana Scovassi
Jul 25, 2014·Journal of Neurology, Neurosurgery, and Psychiatry·Johan A F KoekkoekMartin J B Taphoorn
Feb 11, 2015·Cell Death and Differentiation·M Chiara Maiuri, G Kroemer
Mar 25, 2015·Cell Cycle·Valentina CianfanelliFrancesco Cecconi
May 1, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christoph BurdelskiStefan Steurer

❮ Previous
Next ❯

Citations

Apr 21, 2017·American Journal of Physiology. Renal Physiology·Falguni DasGoutam Ghosh Choudhury
Oct 16, 2019·Journal of Cellular Physiology·Jessica R HonoratoTania C L de S Spohr
Nov 13, 2018·The European Physical Journal. C, Particles and Fields·Angelo Esposito, Antonio D Polosa
Nov 30, 2019·Scientific Reports·Huibin LiuMahmut Mardan
May 29, 2018·Cell Stress & Chaperones·Tambudzai KudzeAmie J McClellan
Dec 11, 2020·Experimental Cell Research·Madison T Wright, Lars Plate
Nov 13, 2020·Cell Death & Disease·Jie-Hui TanGuo-Wei Zhang
Feb 20, 2021·Investigative Ophthalmology & Visual Science·Xiaowen CaoXiangtian Zhou
Oct 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hyeon Ji Kim, Do-Yeon Kim

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
confocal microscopy
xenograft
Assay
gene knockdown
immunoprecipitation assay
protein assay
electrophoresis
Infrared Imaging
ELISA

Software Mentioned

GraphPad
Image J
GraphPad Prism
Living Image
LSM Image Browser

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis