Emergence of a hybrid plasmid derived from IncN1-F33:A-:B- and mcr-1-bearing plasmids mediated by IS26

The Journal of Antimicrobial Chemotherapy
Dan Dan HeGong Zheng Hu

Abstract

To characterize the complete sequences of four plasmids in MCR-1-producing clinical Escherichia coli strain D72, and to depict the formation mechanism and characteristics of the cointegrate plasmid derived from the pD72-mcr1 and pD72-F33 plasmids. The genetic profiles of plasmids in strain D72 and its transconjugant were determined by conjugation, S1-PFGE, Southern hybridization, WGS analysis and PCR. Plasmid sequences were analysed with bioinformatic tools. The traits of the fusion plasmid were characterized by cointegration, stability and conjugation assays. Strain D72, belonging to ST1114, contained four plasmids, including mcr-1-carrying pD72-mcr1, blaCTX-M-55-carrying pD72-F33, blaTEM-238-bearing pD72-IncP and pD72-IncX1 carrying aph(3')-Ia, qnrS2 and floR. A single plasmid, pD72C, in the transconjugant was found to be larger than any plasmid in the original strain D72. Sequence analysis showed that pD72C was the fusion product of pD72-mcr1 and pD72-F33, and the recombinant event involved an intermolecular replicative mechanism. Plasmid fusion occurred at a frequency of 1.75 × 10-4 cointegrates per transconjugant. The fusion plasmid presented a high stability and conjugation frequency of 8.00 × 10-3. To our knowledge, this...Continue Reading

References

Feb 1, 2011·Bioinformatics·Mitchell J SullivanScott A Beatson
Aug 10, 2011·BMC Genomics·Nabil-Fareed AlikhanScott A Beatson
Jul 24, 2013·Antimicrobial Agents and Chemotherapy·David L LinMarcelo E Tolmasky
May 5, 2016·Nucleic Acids Research·David ArndtDavid S Wishart
Sep 14, 2016·Antimicrobial Agents and Chemotherapy·Erik SnesrudFred Dyda
Apr 19, 2017·Antimicrobial Agents and Chemotherapy·Laurent PoirelPatrice Nordmann
Jun 9, 2017·PLoS Computational Biology·Ryan R WickKathryn E Holt
Nov 2, 2017·The Journal of Antimicrobial Chemotherapy·Marcus Ho-Yin WongSheng Chen
Jan 27, 2018·The Journal of Antimicrobial Chemotherapy·Miaomiao XieSheng Chen
Feb 7, 2018·Frontiers in Microbiology·Weilong ZhouZhiyong Zong
Mar 15, 2018·Frontiers in Microbiology·Renjie WuJian-Hua Liu
Feb 13, 2019·FEMS Microbiology Letters·Carol H PongRuth M Hall

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Citations

Jan 21, 2021·Nature Reviews. Microbiology·Jerónimo Rodríguez-BeltránÁlvaro San Millán
Nov 18, 2020·Microorganisms·Andrey ShelenkovVasiliy Akimkin
Oct 7, 2021·Microbiology Spectrum·Yushan PanDandan He

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