PMID: 7517040Jun 21, 1994Paper

Emergence of a replicating species from an in vitro RNA evolution reaction

Proceedings of the National Academy of Sciences of the United States of America
Ronald R. Breaker, G F Joyce

Abstract

The technique of self-sustained sequence replication allows isothermal amplification of DNA and RNA molecules in vitro. This method relies on the activities of a reverse transcriptase and a DNA-dependent RNA polymerase to amplify specific nucleic acid sequences. We have modified this protocol to allow selective amplification of RNAs that catalyze a particular chemical reaction. During an in vitro RNA evolution experiment employing this modified system, a unique class of "selfish" RNAs emerged and replicated to the exclusion of the intended RNAs. Members of this class of selfish molecules, termed RNA Z, amplify efficiently despite their inability to catalyze the target chemical reaction. Their amplification requires the action of both reverse transcriptase and RNA polymerase and involves the synthesis of both DNA and RNA replication intermediates. The proposed amplification mechanism for RNA Z involves the formation of a DNA hairpin that functions as a template for transcription by RNA polymerase. This arrangement links the two strands of the DNA, resulting in the production of RNA transcripts that contain an embedded RNA polymerase promoter sequence.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Dec 1, 1992·Scientific American·G F Joyce
Mar 1, 1992·Trends in Biochemical Sciences·J W Szostak
Aug 1, 1992·PCR Methods and Applications·R C Cadwell, G F Joyce
Jul 31, 1992·Science·A A Beaudry, G F Joyce
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J C GuatelliT R Gingeras
Jun 26, 1989·Nucleic Acids Research·P B Joyce, M W Gray
Nov 11, 1987·Nucleic Acids Research·J F MilliganO C Uhlenbeck
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·C K Biebricher, L E Orgel
Jul 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·D R MillsS Spiegelman
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P DavanlooF W Studier
Jun 5, 1983·Journal of Molecular Biology·J J Dunn, F W Studier

❮ Previous
Next ❯

Citations

Jul 7, 2005·Journal of Molecular Evolution·Glenn C Johns, Gerald F Joyce
Apr 23, 2003·Biosensors & Bioelectronics·Yi LuAndrea K Brown
Jan 13, 1998·Current Opinion in Biotechnology·P A PattenW P Stemmer
Mar 28, 2002·Journal of Biotechnology·G MayerJ M Köhler
Aug 16, 2012·Accounts of Chemical Research·Adam J MeyerAndrew D Ellington
Oct 4, 2013·Nature Communications·Norikazu IchihashiTetsuya Yomo
May 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Bryan C DickinsonDavid R Liu
Aug 13, 2008·Nucleosides, Nucleotides & Nucleic Acids·B J BoeseR R Breaker
Jan 15, 1996·European Journal of Biochemistry·M Gebinoga, F Oehlenschläger
Jan 15, 1997·European Journal of Biochemistry·R EhrichtJ S McCaskill
Jan 1, 1997·Annual Review of Biochemistry·G J Narlikar, D Herschlag
Jan 31, 2008·RNA·Ulrich F Müller, David P Bartel
Nov 13, 2013·Nucleic Acids Research·Wadim L MatochkoRatmir Derda
Nov 5, 2013·Nature Biotechnology·Jared W EllefsonAndrew D Ellington
May 29, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tomoaki MatsuuraTetsuya Yomo
Oct 11, 2013·BMC Biotechnology·Hidenao AraiYuzuru Husimi
Jun 1, 1997·Current Biology : CB·M P Robertson, A D Ellington
Jan 1, 1997·Chemistry & Biology·B Wlotzka, J S McCaskill
Apr 23, 2008·Current Protocols in Nucleic Acid Chemistry·S D Jhaveri, A D Ellington
Apr 23, 2008·Current Protocols in Nucleic Acid Chemistry·R R Breaker
Jan 20, 2018·Physical Review. E·Harold FellermannSteen Rasmussen
Feb 23, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ratmir DerdaMohammad R Jafari
Jul 20, 2007·Angewandte Chemie·Gerald F Joyce
Apr 9, 2019·Annals of the New York Academy of Sciences·Norikazu Ichihashi
Mar 5, 2010·Current Protocols in Nucleic Acid Chemistry·Bradley HallAndrew D Ellington
Mar 5, 2010·Current Protocols in Nucleic Acid Chemistry·Carolina Díaz Arenas, Niles Lehman
Feb 12, 2008·Current Protocols in Molecular Biology·S D Jhaveri, A D Ellington
Oct 10, 2009·Current Protocols in Molecular Biology·Bradley HallAndrew D Ellington
Aug 10, 2018·Scientific Reports·Tomoaki YoshiyamaNorikazu Ichihashi
Nov 13, 2019·Bio Systems·Athel Cornish-Bowden, María Luz Cárdenas
Oct 22, 2020·Synthetic and Systems Biotechnology·Wenhui Zhang, Qiong Wu
Apr 1, 1997·Chemical Reviews·Ronald R. Breaker
May 9, 2013·ACS Synthetic Biology·Eric A DavidsonAndrew D Ellington
Aug 10, 2007·Bioorganic & Medicinal Chemistry·Tatiana V AbramovaVladimir N Silnikov
Aug 19, 1994·Science·F Flam
Apr 25, 1997·Science·M C Wright, G F Joyce
Apr 25, 1997·Science·A D EllingtonJ Bull

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.