Emergence of Aspergillus fumigatus azole resistance in azole-naïve patients with chronic obstructive pulmonary disease and their homes

Indoor Air
C DauchyE Fréalle

Abstract

Azole-resistant Aspergillus fumigatus (ARAF) has been reported in patients with chronic obstructive pulmonary disease (COPD) but has not been specifically assessed so far. Here, we evaluated ARAF prevalence in azole-naïve COPD patients and their homes, and assessed whether CYP51A mutations were similar in clinical and environmental reservoirs. Sixty respiratory samples from 41 COPD patients with acute exacerbation and environmental samples from 36 of these patient's homes were prospectively collected. A. fumigatus was detected in respiratory samples from 11 of 41 patients (27%) and in 15 of 36 domiciles (42%). Cyp51A sequencing and selection on itraconazole medium of clinical (n = 68) and environmental (n = 48) isolates yielded ARAF detection in 1 of 11 A. fumigatus colonized patients with COPD (9%) and 2 of 15 A. fumigatus-positive patient's homes (13%). The clinical isolate had no CYP51A mutation. Two environmental isolates from two patients harbored TR34 /L98H mutation, and one had an H285Y mutation. Coexistence of different cyp51A genotypes and/or azole resistance profiles was detected in 3 of 8 respiratory and 2 of 10 environmental samples with more than one isolate, confirming the need for a systematic screening of all cl...Continue Reading

References

Dec 5, 1993·Journal of Molecular Biology·A Sali, T L Blundell
Mar 28, 2002·Applied and Environmental Microbiology·Brian G SheltonGeorge K Morris
Oct 3, 2008·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·UNKNOWN Subcommittee on Antifungal Susceptibility Testing of the ESCMID European Committee for Antimicrobial Susceptibility Testing
Apr 21, 2009·Applied and Environmental Microbiology·Eveline SneldersPaul E Verweij
Jul 25, 2009·Emerging Infectious Diseases·Susan J HowardDavid W Denning
Oct 21, 2009·Indoor Air·G RebouxL Millon
Nov 13, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J GuineaE Bouza
Apr 14, 2010·Antimicrobial Agents and Chemotherapy·Eveline SneldersWillem J G Melchers
Aug 24, 2010·The Journal of Antimicrobial Chemotherapy·Ahmed BueidDavid W Denning
Sep 2, 2010·Antimicrobial Agents and Chemotherapy·Klaus Leth MortensenMaiken Cavling Arendrup
Oct 18, 2011·Emerging Infectious Diseases·Jan W M van der LindenPaul E Verweij
Oct 26, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·H XuW-F Yuan
Jun 20, 2012·Antimicrobial Agents and Chemotherapy·Alexandre AlanioStéphane Bretagne
Apr 20, 2013·The European Respiratory Journal·Mona BafadhelChristopher E Brightling
May 15, 2013·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jan W M van der LindenPaul E Verweij
Aug 27, 2013·The Science of the Total Environment·Emeline SchererLaurence Millon
Apr 5, 2014·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·A PrigitanoA M Tortorano
Aug 3, 2014·The Journal of Antimicrobial Chemotherapy·Isabel MontesinosOlivier Denis
Oct 19, 2014·The Journal of Antimicrobial Chemotherapy·Suhail AhmadZiauddin Khan
Apr 29, 2015·Antimicrobial Agents and Chemotherapy·Rose-Anne LavergnePatrice Le Pape
May 6, 2015·Antimicrobial Agents and Chemotherapy·Oliver BaderUwe Groß
May 20, 2015·Antimicrobial Agents and Chemotherapy·Edith VermeulenKatrien Lagrou
Jun 21, 2015·Fungal Genetics and Biology : FG & B·Eveline SneldersWillem J G Melchers
Jul 15, 2015·The Journal of Antimicrobial Chemotherapy·J FuhrenJ G Kusters
Aug 19, 2015·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Paul E VerweijJ Peter Donnelly
Dec 4, 2015·Critical Care : the Official Journal of the Critical Care Forum·Claire DelsucFlorence Ader
Dec 23, 2015·Emerging Infectious Diseases·Alexandre AlanioStéphane Bretagne
Apr 16, 2016·American Journal of Respiratory and Critical Care Medicine·Eva KolwijckPaul E Verweij
Aug 6, 2016·The Journal of Antimicrobial Chemotherapy·Paul E VerweijJacques F Meis
Sep 30, 2016·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rose-Anne LavergnePatrice Le Pape
Apr 6, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sang Taek HeoDimitrios P Kontoyiannis

❮ Previous
Next ❯

Citations

Feb 3, 2018·Environmental Microbiology·Anuradha Chowdhary, Jacques F Meis
Apr 10, 2019·Proceedings. Biological Sciences·Jianhua ZhangAlfons J M Debets
Jun 27, 2019·Antimicrobial Agents and Chemotherapy·Thomas R SewellMatthew C Fisher
Dec 24, 2018·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P P A LestradeP E Verweij
Mar 7, 2021·Journal of Fungi·Mireille H van der TorreLilyann Novak-Frazer
Jun 17, 2021·Pulmonology·Chris KosmidisJørgen Vestbo
Sep 8, 2021·Mycopathologia·Amelie P BrackinJohanna Rhodes
Dec 7, 2021·Microbiology and Immunology·Sofia Marisel Rivelli Zea, Takahito Toyotome

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.