Emergence of vancomycin-resistant Enterococcus faecium ST1421 lacking the pstS gene in Korea.

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
Hye Mee KimKyong Ran Peck

Abstract

Although multilocus sequence typing (MLST) has been used to study molecular epidemiology and to explore the population structure of Enterococcus faecium, vancomycin-resistant E. faecium (VREF) strains lacking the pstS gene that were non-typable using conventional MLST methods were reported recently. We found nationwide emergence of VREF isolates lacking pstS in Korea and hereby report the molecular characteristics of these isolates. Forty-six VREF isolates lacking the pstS gene were identified among 300 VREF rectal isolates collected from hospitalized patients between 2014 and 2015. MLST was performed and clonal relatedness was determined by pulsed-field gel electrophoresis (PFGE). Four VREF ST1421 isolates were whole-genome sequenced. Among the VREF rectal isolates lacking pstS, 98% were classified as ST1421, which has identical allelic profiles to ST17 for all housekeeping genes except pstS. PFGE pattern analyses revealed 32 pulsotypes. All isolates harbored Tn1546 components with various transposase and insertion sequences. The whole-genome sequencing of four VREF ST1421 isolates showed that the pstS gene region was deleted at various locations with considerable inversion. The pstS gene was also depleted in 12.1% of 33 VREF ...Continue Reading

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Citations

Aug 7, 2021·Microbial Genomics·Jordy P M Coolen On Behalf Of Sig Bioinformatics In Medical Microbiology Nl Consortium

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Methods Mentioned

BETA
PCR
electrophoresis
genotyping

Software Mentioned

BIONJ
BLAST
FigTree
MUSCLE
Mauve genome alignment viewer
SMRT
BLAST Ring Image Generator ( BRIG )
BPGA
SMRT Analysis

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