The role of geometrical confinement on collective cell migration has been recognized but has not been elucidated yet. Here, we show that the geometrical properties of the environment regulate the formation of collective cell migration patterns through cell-cell interactions. Using microfabrication techniques to allow epithelial cell sheets to migrate into strips whose width was varied from one up to several cell diameters, we identified the modes of collective migration in response to geometrical constraints. We observed that a decrease in the width of the strips is accompanied by an overall increase in the speed of the migrating cell sheet. Moreover, large-scale vortices over tens of cell lengths appeared in the wide strips whereas a contraction-elongation type of motion is observed in the narrow strips. Velocity fields and traction force signatures within the cellular population revealed migration modes with alternative pulling and/or pushing mechanisms that depend on extrinsic constraints. Force transmission through intercellular contacts plays a key role in this process because the disruption of cell-cell junctions abolishes directed collective migration and passive cell-cell adhesions tend to move the cells uniformly toget...Continue Reading
Multiple rows of cells behind an epithelial wound edge extend cryptic lamellipodia to collectively drive cell-sheet movement
A steering model of endothelial sheet migration recapitulates monolayer integrity and directed collective migration
Two-photon laser-generated microtracks in 3D collagen lattices: principles of MMP-dependent and -independent collective cancer cell invasion
A photoactivatable nanopatterned substrate for analyzing collective cell migration with precisely tuned cell-extracellular matrix ligand interactions
Proteolytic and non-proteolytic regulation of collective cell invasion: tuning by ECM density and organization
Phototriggered fibril-like environments arbitrate cell escapes and migration from endothelial monolayers
A historical to present-day account of efforts to answer the question: "what puts the brakes on mammalian hair cell regeneration?"
Probing Leader Cells in Endothelial Collective Migration by Plasma Lithography Geometric Confinement
Aqueous biphasic cancer cell migration assay enables robust, high-throughput screening of anti-cancer compounds
The formation of ordered nanoclusters controls cadherin anchoring to actin and cell-cell contact fluidity
Matrix-Immobilized BMP-2 on Microcontact Printed Fibronectin as an in vitro Tool to Study BMP-Mediated Signaling and Cell Migration
Collective migration exhibits greater sensitivity but slower dynamics of alignment to applied electric fields
N-Cadherin and Fibroblast Growth Factor Receptors crosstalk in the control of developmental and cancer cell migrations
Dynamic myosin activation promotes collective morphology and migration by locally balancing oppositional forces from surrounding tissue
Quantitative Live Imaging of Human Embryonic Stem Cell Derived Neural Rosettes Reveals Structure-Function Dynamics Coupled to Cortical Development
Microfabricated tissues for investigating traction forces involved in cell migration and tissue morphogenesis
Dynamics of Cell Ensembles on Adhesive Micropatterns: Bridging the Gap between Single Cell Spreading and Collective Cell Migration
Facile preparation of a photoactivatable surface on a 96-well plate: a versatile and multiplex cell migration assay platform
Inhibition of cell-cell adhesion impairs directional epithelial migration on micropatterned surfaces
Celebrating Soft Matter's 10th Anniversary: Cell division: a source of active stress in cellular monolayers
Direction-dependent contraction forces on cell boundaries induce collective migration of epithelial cells within their sheet
Force-dependent binding of vinculin to α-catenin regulates cell-cell contact stability and collective cell behavior
Dynamic control of cell adhesion on a stiffness-tunable substrate for analyzing the mechanobiology of collective cell migration
Stereolithographic printing of ionically-crosslinked alginate hydrogels for degradable biomaterials and microfluidics
Multicompartment cell-based modeling of confined migration: regulation by cell intrinsic and extrinsic factors
The significance of tumour microarchitectural features in breast cancer prognosis: a digital image analysis
Energetic regulation of coordinated leader-follower dynamics during collective invasion of breast cancer cells
Interplay Between the Persistent Random Walk and the Contact Inhibition of Locomotion Leads to Collective Cell Behaviors
A Facile Assay of Epithelial-mesenchymal Transition Based on Cooperativity Quantification of Cellular Autonomous Motions
A practical guide to active colloids: choosing synthetic model systems for soft matter physics research
Local cell coordination does not alter individual cell migration during collective migration but does impact cellular exchange events
Collective cell traction force analysis on aligned smooth muscle cell sheet between three-dimensional microwalls
Switch-like enhancement of epithelial-mesenchymal transition by YAP through feedback regulation of WT1 and Rho-family GTPases
Direct cellular organization with ring-shaped composite polymers and glass substrates for urethral sphincter tissue engineering
Advanced capability of radially aligned fibrous scaffolds coated with polydopamine for guiding directional migration of human mesenchymal stem cells
Azobenzene-based sinusoidal surface topography drives focal adhesion confinement and guides collective migration of epithelial cells.
Defining Cell Cluster Size by Dielectrophoretic Capture at an Array of Wireless Electrodes of Several Distinct Lengths
Epithelial vertex models with active biochemical regulation of contractility can explain organized collective cell motility
Femtosecond laser induced nano-textured micropatterning to regulate cell functions on implanted biomaterials.
Study on the Expansion Dynamics of MDCK Epithelium by Interstitial Flow Using a Traction Force-Measurable Microfluidic Chip.
A junctional PACSIN2/EHD4/MICAL-L1 complex coordinates VE-cadherin trafficking for endothelial migration and angiogenesis.
Methodology for comprehensive cell-level analysis of wound healing experiments using deep learning in MATLAB.
Distinct Modes of Tissue Expansion in Free Versus Earlier-Confined Boundaries for More Physiological Modeling of Wound Healing, Cancer Metastasis, and Tissue Formation.
Adhesion Molecules in Health and Disease
Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.
Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.
Biophysics of Adhesion
Alterations in cell adhesion can disrupt important cellular processes and lead to a variety of diseases, including cancer and arthritis. It is also essential for infectious organisms, such as bacteria or viruses, to cause diseases. Understanding the biophysics of cell adhesion can help understand these diseases. Discover the latest research on the biophysics of adhesion here.