Emerging technologies for profiling extracellular vesicle heterogeneity.

Lab on a Chip
Guan HuangDayong Jin

Abstract

Extracellular vesicles (EVs) are membrane-bound vesicles secreted by most cell types and exist in virtually all bodily fluids. They carry on a wealth of proteomic and genetic information including proteins, lipids, miRNAs, mRNA, non-coding RNA and other molecules from parental cells. Increasing evidence shows that within populations of EVs, their biogenesis, physical characteristics (e.g. size, density, morphology) and cargos (e.g. protein, lipid content, nucleic acids) may vary substantially, which accordingly change their biological properties. To fully exploit the potential of EVs, it requires qualified methods to profile EV heterogeneity. In this review, we survey recent approaches for EV isolation with innovative discoveries in heterogeneity. The main challenges in EV heterogeneity research are identified, and the roles of single cell EV profiling and single EV imaging are highlighted. We further discuss promising opportunities for resolving the underlying complexity of EV heterogeneity.

References

Aug 30, 1990·Nature·A D Ellington, J W Szostak
May 1, 2002·Nature Biotechnology·Simon FredrikssonUlf Landegren
Aug 15, 2003·Nature·David G Grier
Jan 14, 2004·British Journal of Haematology·Hyun Kyung KimSe-Na Lee
Apr 23, 2004·Current Opinion in Biotechnology·Helene Andersson, Albert van den Berg
Aug 25, 2004·Proceedings of the National Academy of Sciences of the United States of America·Trairak PisitkunMark A Knepper
Jul 28, 2006·Nature·George M Whitesides
Jul 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Charlotte AdmyreSusanne Gabrielsson
Jan 30, 2008·Current Protocols in Cell Biology·Clotilde ThéryAled Clayton
Jun 6, 2009·Annual Review of Biochemistry·Bo HuangXiaowei Zhuang
Apr 9, 2010·Nature·Robert C R Wootton, Andrew J Demello
Jul 21, 2010·The Journal of Cell Biology·Lothar SchermellehHeinrich Leonhardt
Jan 18, 2011·Journal of Translational Medicine·Cecilia LässerHadi Valadi
Apr 14, 2011·Chemical Society Reviews·Maureen A Walling, Jason R E Shepard
Jul 7, 2012·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bradley M ColemanAndrew F Hill
Jan 24, 2014·Journal of Extracellular Vesicles·Yuana YuanaSusanne Osanto
Jan 28, 2014·Journal of Ovarian Research·Allison BeachSham S Kakar
Feb 4, 2014·Current Opinion in Biotechnology·Westbrook M WeaverDino Di Carlo
Mar 13, 2014·Journal of Thrombosis and Haemostasis : JTH·N ArraudA R Brisson
Mar 14, 2014·Nature·Eric K SackmannDavid J Beebe
May 29, 2014·Nature Communications·Tzviya Zeev-Ben-MordehaiKay Grünewald
Aug 28, 2014·Cell Reports·Mario Leonardo SquadritoMichele De Palma
Sep 11, 2014·Journal of Extracellular Vesicles·Marijke I ZonneveldEsther N M Nolte-'t Hoen
Jan 27, 2015·Chemical Society Reviews·Otto S Wolfbeis
May 7, 2015·Oncotarget·Shivakumar KeerthikumarSuresh Mathivanan
Jul 2, 2015·ELife·Diana J ChaJames G Patton
Aug 12, 2015·Nature Communications·Marina MikhaylovaLukas C Kapitein
Sep 22, 2015·Cell Reports·Guillaume van NielGraça Raposo
Oct 21, 2015·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Samuel A StonerJohn P Nolan
Nov 5, 2015·Nature Nanotechnology·Bo ZhouXiaogang Liu
Nov 14, 2015·Journal of Extracellular Vesicles·Johanna L Höög, Jan Lötvall
Feb 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Joanna KowalClotilde Théry
Feb 24, 2016·Journal of Extracellular Vesicles·Nina KolihaStefan Wild
Mar 5, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Scott W Ferguson, Juliane Nguyen
Sep 30, 2016·Journal of Extracellular Vesicles·Brian MeehanDolores Di Vizio

❮ Previous
Next ❯

Citations

Dec 22, 2020·Small·Michael J SiedlikDavid Issadore
Dec 9, 2020·Biosensors & Bioelectronics·Guocheng FangDayong Jin
Mar 21, 2021·Analytical Biochemistry·Teran CloetHuiyan Li
May 1, 2021·International Journal of Molecular Sciences·Maja KosanovićMilica Bozic
Jul 3, 2021·International Journal of Molecular Sciences·Giulia CricrìFederica Collino
May 30, 2021·Tissue Engineering and Regenerative Medicine·Dong Jun Park, Young Joon Seo
Aug 14, 2021·Journal of Nanobiotechnology·Yong-Jiang LiDa-Xiong Xiang
Dec 3, 2021·Nano-micro Letters·Cheng JiangGeorge K Tofaris
Dec 9, 2021·ACS Sensors·Gungun Lin, Dayong Jin

❮ Previous
Next ❯

Methods Mentioned

BETA
electron microscopy
flow cytometry
size-exclusion chromatography
chip
flow
light scattering
PCR
ELISA
Fluorescence
chips

Software Mentioned

HSFCM
STORM

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.