Emerging technologies for studying DNA methylation for the molecular diagnosis of cancer

Expert Review of Molecular Diagnostics
Diego M Marzese, Dave Sb Hoon

Abstract

DNA methylation is an epigenetic mechanism that plays a key role in regulating gene expression and other functions. Although this modification is seen in different sequence contexts, the most frequently detected DNA methylation in mammals involves cytosine-guanine dinucleotides. Pathological alterations in DNA methylation patterns are described in a variety of human diseases, including cancer. Unlike genetic changes, DNA methylation is heavily influenced by subtle modifications in the cellular microenvironment. In all cancers, aberrant DNA methylation is involved in the alteration of a large number of oncological pathways with relevant theranostic utility. Several technologies for DNA methylation mapping have been developed recently and successfully applied in cancer studies. The scope of these technologies varies from assessing a single cytosine-guanine locus to genome-wide distribution of DNA methylation. Here, we review the strengths and weaknesses of these approaches in the context of clinical utility for the molecular diagnosis of human cancers.

References

Jul 20, 1987·Journal of Molecular Biology·M Gardiner-Garden, M Frommer
Jun 26, 1981·Biochimica Et Biophysica Acta·I Wagner, I Capesius
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·W H LeeW G Nelson
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·J G HermanS B Baylin
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·S A BelinskyJ G Herman
Mar 2, 1999·Journal of the National Cancer Institute·S A AhrendtD Sidransky
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·M ToyotaJ P Issa
Mar 29, 2000·Nucleic Acids Research·C A EadsP W Laird
Aug 30, 2000·Nature·C M PerouD Botstein
Sep 11, 2001·The American Journal of Pathology·A RashidS R Hamilton
Nov 16, 2001·Annals of the New York Academy of Sciences·C GoesslK Miller
Dec 26, 2001·Human Molecular Genetics·M EstellerJ G Herman
Nov 25, 2003·The New England Journal of Medicine·James G Herman, Stephen B Baylin
Apr 3, 2004·Molecular and Cellular Biology·Helle F JørgensenAdrian P Bird
Jul 9, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Maria PelliséJosep M Piqué
Oct 7, 2004·Nature Structural & Molecular Biology·Matthew C LorinczMark Groudine
Nov 19, 2004·European Urology·Patrick J BastianWilliam G Nelson
Dec 1, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Naoyuki UmetaniDave S B Hoon
Mar 11, 2005·The New England Journal of Medicine·Monika E HegiRoger Stupp
Mar 25, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Masaru ShinozakiBret Taback
Oct 15, 2005·Nucleic Acids Research·Alexander MeissnerRudolf Jaenisch
Dec 20, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Takuji MoriDave S B Hoon
Dec 22, 2005·Nature Reviews. Genetics·Andrew P FeinbergSteven Henikoff
Jan 13, 2006·Oncogene·I HatadaH Sasaki
Jan 27, 2006·The Journal of Molecular Diagnostics : JMD·Edward J FoxKieran Sheahan
May 12, 2006·Molecular Cancer Research : MCR·Naoyuki UmetaniDave S B Hoon
Jul 1, 2006·Genome Research·Batbayar KhulanJohn M Greally
Jul 5, 2006·Cancer Research·Takuji MoriDave S B Hoon
Jul 11, 2006·The New England Journal of Medicine·Arlo J Miller, Martin C Mihm
Nov 17, 2006·Annals of the New York Academy of Sciences·Bret TabackDave S B Hoon
Jan 16, 2007·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Debra Ting HsiungKarl T Kelsey
Feb 24, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Christian Adrien RighiniMarie-Christine Favrot

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Citations

Jan 8, 2016·Clinical Chemistry and Laboratory Medicine : CCLM·Ioanna BalgkouranidouEvi Lianidou
Oct 27, 2016·Revista chilena de pediatría·Paola CasanelloRicardo Uauy
Jan 19, 2018·Science Translational Medicine·Helen R MoinovaSanford D Markowitz
May 22, 2019·Stem Cell Research & Therapy·Stefania TrinoLuciana De Luca
Jul 11, 2019·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Xi JiaJinsong Hu
Jan 17, 2019·Mediators of Inflammation·Jose Luis Morgado-PascualSandra Rayego-Mateos
Dec 6, 2018·Journal of Cellular Physiology·Rana ShafabakhshZatollah Asemi
Aug 14, 2021·Frontiers in Genetics·Megan E BarefootAnton Wellstein

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