Emodin alleviated pulmonary inflammation in rats with LPS-induced acute lung injury through inhibiting the mTOR/HIF-1α/VEGF signaling pathway

Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]
Xiaoqian LiBo Tan

Abstract

This study aimed to investigate the anti-pulmonary inflammation effect of emodin on Wistar rats with lipopolysaccharide (LPS)-induced acute lung injury (ALI) and RAW264.7 cells through the mammalian target of rapamycin (mTOR)/hypoxia-inducible factor-1α (HIF-1α)/vascular endothelial growth factor (VEGF) signaling pathway. Wistar rats and RAW264.7 cells were studied. LPS was used to induce inflammation in rats or RAW264.7 cells and emodin was given once a day before LPS stimulation and continued for a certain number of days. Lung tissues and bronchoalveolar lavage fluid (BALF) were collected for the in vivo experiment, while cells and supernatant were collected for the in vitro experiment. Pathological changes in the lung tissues were assessed by hematoxylin and eosin staining. The levels of inflammatory factors, including TNF-α, IL-1β, and IL-6, were determined by enzyme-linked immunosorbent assay. The expression levels of p-mTOR, HIF-1α, and VEGF proteins were measured by Western blot analysis and immunohistochemistry. The mRNA levels of p70S6K, eIF4E-BP1, and eIF4E were measured by quantitative polymerase chain reaction. Emodin ameliorated pathological changes and infiltrated inflammatory cells in LPS-induced ALI. It also sig...Continue Reading

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Citations

Oct 3, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Yu-Qiong HeWan-Sheng Chen
Dec 8, 2020·Oxidative Medicine and Cellular Longevity·Yang LiuYanfeng Wang
Aug 10, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xianfeng LiuXianli Meng
Nov 14, 2021·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Jia LiXiaoling Liang

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Methods Mentioned

BETA
bronchoalveolar
enzyme-linked immunosorbent assay
PCR
bronchoalveolar lavage

Software Mentioned

AlphaView SA
Image Analysis System
Medical Image Analysis
SPSS18

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