Emodin ameliorates cisplatin-induced apoptosis of rat renal tubular cells in vitro by activating autophagy

Acta Pharmacologica Sinica
Hong LiuWei Sun

Abstract

A previous report shows that emodin extracted from the Chinese herbs rhubarb and giant knotweed rhizome can ameliorate the anticancer drug cisplatin-induced injury of HEK293 cells. In this study, we investigated whether and how emodin could protect renal tubular epithelial cells against cisplatin-induced nephrotoxicity in vitro. The viability and apoptosis of normal rat renal tubular epithelial cells (NRK-52E) were detected using formazan assay and flow cytometry analysis, respectively. The expression levels of cleaved caspase-3, autophagy maker LC3 I/II, and AMPK/mTOR signaling pathway-related proteins were measured with Western blot analysis. The changes of morphology and RFP-LC3 fluorescence were observed under microscopy. Cisplatin (10-50 μmol/L) dose-dependently induced cell damage and apoptosis in NRK-52E cells, whereas emodin (10 and 100 μmol/L) significantly ameliorated cisplatin-induced cell damage, apoptosis and caspase-3 cleavage. Emodin dose-dependently increased LC3-II levels and induced RFP-LC3-containing punctate structures in NRK-52E cells. Furthermore, the protective effects of emodin were abolished by bafilomycin A1 (10 nmol/L), and mimicked by rapamycin (100 nmol/L). Moreover, emodin increased the phosphoryla...Continue Reading

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Citations

Oct 25, 2016·Oxidative Medicine and Cellular Longevity·Shreesh OjhaMohanraj Rajesh
Jan 22, 2020·Journal of Cellular Physiology·Yingyu ChenJianda Hu
Dec 20, 2017·Molecular Medicine Reports·Haoyuan JiaHui Qian
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Jul 1, 2021·Néphrologie & thérapeutique·Yue QiuChun Zhang

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