Emodin down-regulates expression of TRPV1 mRNA and its function in DRG neurons in vitro

The American Journal of Chinese Medicine
Feng SuiTingliang Jiang

Abstract

Emodin is a principle ingredient isolated from rhubarb rhizome, which is commonly used for constipation or pain-related diseases in traditional Chinese medicine (TCM) practice. The transient receptor potential vanilloid 1 ion channel proteins (TRPV1) are abundantly expressed in the peripheral sensory neurons and are assumed to act as a kind of nociceptor involved in the perception of pain and development of hyperalgesia. The aim of this study was to further unravel the analgesic mechanisms of rhubarb through investigating the effects of its main constitutive ingredient emodin on the expression of TRPV1 mRNA as well as on its calcium- mediating functions in vitro. The primary DRG neurons with a high purity and viability were obtained, and the TRPV1 mRNA expression levels were examined by using real-time RT-PCR and the elevated amplitudes of intracellular [Ca(2+)]i in the DRG neurons evoked by TRPV1 agonist capsaicin were examined by confocal microscopy. The results showed that emodin could significantly down-regulate both the mRNA expression of TRPV1 and the capsaicin-evoked intracellular fluorescent intensity in the DRG neurons under both 37 degrees C and 39 degrees C in vitro. Concomitantly, all of the changes induced by emodi...Continue Reading

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Citations

Aug 13, 2011·Drug and Chemical Toxicology·Qiuxia HeXimin Wang
May 17, 2018·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Ajeet Kumar SinghManjula Vinayak
Feb 6, 2021·Frontiers in Pharmacology·Juan LiuCheng Peng
Jul 27, 2021·Phytochemistry·Ruchi Badoni SemwalAlvaro Viljoen

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