Emodin enhances the demethylation by 5-Aza-CdR of pancreatic cancer cell tumor-suppressor genes P16, RASSF1A and ppENK

Oncology Reports
Feng-Ping PanLiang Chen

Abstract

5-Aza-2'-deoxycytidine (5-Aza-CdR) is currently acknowledged as a demethylation drug, and causes a certain degree of demethylation in a variety of cancer cells, including pancreatic cancer cells. Emodin, a traditional Chinese medicine (TCM), is an effective monomer extracted from rhubarb and has been reported to exhibit antitumor activity in different manners in pancreatic cancer. In the present study, we examined whether emodin caused demethylation and increased the demethylation of three tumor-suppressor genes P16, RASSF1A and ppENK with a high degree of methylation in pancreatic cancer when combined with 5-Aza-CdR. Our research showed that emodin inhibited the growth of pancreatic cancer Panc-1 cells in a dose- and time-dependent manner. Dot-blot results showed that emodin combined with 5-Aza-CdR significantly suppressed the expression of genome 5mC in PANC-1 cells. In order to verify the effect of methylation, methylation-specific PCR (MSP) and bisulfite genomic sequencing PCR (BSP) combined with TA were selected for the cloning and sequencing. Results of MSP and BSP confirmed that emodin caused faint demethylation, and 5-Aza-CdR had a certain degree of demethylation. When emodin was combined with 5-Aza-CdR, the demethylati...Continue Reading

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Citations

Oct 19, 2017·International Journal of Molecular Sciences·Reinhard H DammannChamindri Witharana
Nov 9, 2018·The International Journal of Neuropsychopharmacology·Peng ZengQing Tian
Jan 30, 2020·Natural Product Reports·Levi W EvansBradley S Ferguson
Oct 25, 2017·Chinese Journal of Integrative Medicine·Meng LiPei-Tong Zhang
Aug 2, 2018·Molecular Medicine Reports·Nan LiShengyi You
Aug 31, 2020·Chinese Medicine·Hong XiangDeshi Dong
Jan 23, 2020·Cancers·Lucía García-GutiérrezDavid Matallanas
Jun 5, 2021·Nutrition and Cancer·Mustafa B A Djamgoz, Valerie Jentzsch

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Methods Mentioned

BETA
surgical resection
PCR
Dot-blot
electrophoresis
ELISA
protein assay
X-ray
transfection

Software Mentioned

BiQ Analyzer
SPSS
ImageJ

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