Emodin promoted pancreatic claudin-5 and occludin expression in experimental acute pancreatitis rats.

World Journal of Gastroenterology : WJG
Xian-Ming XiaHai-Ling Ruan

Abstract

To investigate the effect of emodin on pancreatic claudin-5 and occludin expression, and pancreatic paracellular permeability in acute pancreatitis (AP). Experimental pancreatitis was induced by retrograde injection of 5% sodium taurocholate into the biliopancreatic duct. Emodin was injected via the external jugular vein 0 or 6 h after induction of AP. Rats from sham operation and AP groups were injected with normal saline at the same time. Samples of pancreas were obtained 6 or 12 h after drug administration. Pancreatic morphology was examined with hematoxylin and eosin staining. Pancreatic edema was estimated by measuring tissue water content. Tumor necrosis factor (TNF)-α and interleukin (IL)-6 level were measured by enzyme-linked immunosorbent assay. Pancreatic paracellular permeability was assessed by tissue dye extravasation. Expression of pancreatic claudin-5 and occludin was examined by immunohistology, quantitative real-time reverse transcriptase polymerase chain reaction and western blotting. Pancreatic TNF-α and IL-6 levels, wet/dry ratio, dye extravasation, and histological score were significantly elevated at 3, 6 and 12 h following sodium taurocholate infusion; treatment with emodin prevented these changes at all ...Continue Reading

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Citations

Jul 4, 2017·Acta chirurgica Belgica·Chris BraumannStephan Rogalla
Jun 15, 2017·World Journal of Gastroenterology : WJG·Jun LiZong-Fang Li
Apr 25, 2018·Evidence-based Complementary and Alternative Medicine : ECAM·Laura GamanMarilena Gilca
Aug 25, 2018·Journal of Gastroenterology·Takeshi SatoShin Maeda
Jul 27, 2021·Phytochemistry·Ruchi Badoni SemwalAlvaro Viljoen

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