Emodin protects H9c2 cells from hypoxia-induced injury by up-regulating miR-138 expression

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
Xuezhi ZhangJun Guan

Abstract

Myocardial infarction (MI) is a common presentation for ischemic heart disease, which is a leading cause of death. Emodin is a Chinese herbal anthraquinone used in several diseases. However, the effect of emodin in hypoxia-induced injury in cardiomyocytes has not been clearly elucidated. Our study aimed to clarify the functions of emodin in hypoxia-induced injury in rat cardiomyocytes H9c2 and explore the underlying mechanism. The effects of emodin on cell viability and apoptosis were analyzed by the Cell counting kit-8 assay and flow cytometry assay, respectively. The cell proliferation- and cell apoptosis-related proteins were detected by western blot. qRT-PCR was used to determine the relative expression of miR-138. Cell transfection was performed to alter miR-138 and MLK3 expression. miR-138 target was performed by dual luciferase activity assay. Sirt1/AKT and Wnt/β-catenin pathways-related factors phosphorylation were analyzed by western blot. Emodin inhibited hypoxia-induced injury in H9c2 cells by promoting cell viability and reducing cell apoptosis. miR-138 was down-regulated by hypoxia treatment but up-regulated by emodin. Up-regulation of miR-138 alleviated hypoxia-induced cell injury. Down-regulation of miR-138 atten...Continue Reading

References

Dec 16, 1993·Nature·Y XiongD Beach
May 1, 1993·Genes & Development·V BaldinG Draetta
May 15, 1997·Nature·Y HauptM Oren
Apr 27, 2000·Virchows Archiv : an International Journal of Pathology·I K Bukholm, J M Nesland
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Sep 6, 2007·Cell Cycle·Michael CarletonPeter S Linsley
Nov 10, 2009·Biochemical and Biophysical Research Communications·Jing AiBaofeng Yang
Oct 29, 2010·Journal of Cardiovascular Pharmacology·Fadi N SalloumRakesh C Kukreja
Jun 10, 2011·Trends in Molecular Medicine·Jakob Lewin Rukov, Noam Shomron
Jun 18, 2011·Gastroenterology·Nadja LehwaldKarl G Sylvester
Mar 15, 2013·The Biochemical Journal·Shanshan LiDaling Zhu
Nov 12, 2013·Biochemical and Biophysical Research Communications·Siyi HeYingbin Xiao
Jan 18, 2014·Circulation Research·Susmita Sahoo, Douglas W Losordo
Dec 2, 2014·The American Journal of Medicine·Anoop S V ShahNicholas L Mills
Mar 22, 2016·Experimental and Therapeutic Medicine·Xiang-Jun TangL I Zhang
Sep 28, 2016·Advances in Experimental Medicine and Biology·B Anu MonishaAshu Bhan Tiku
Nov 24, 2016·International Journal of Biological Sciences·Jianming WuZhirong Zhang
Jul 6, 2017·Molecular Medicine Reports·Lu ZhouJung Ryul Kim
Oct 24, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jian-Ping DaiKang-Sheng Li
Jan 2, 2018·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Sheng-Lan TianQing-Bang Xu
Jan 23, 2018·The International Journal of Biochemistry & Cell Biology·Ejazul HaqueSnober S Mir
Mar 27, 2018·Molecular and Cellular Endocrinology·Lei FanTao Liu

❮ Previous
Next ❯

Citations

Jul 23, 2020·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Fenghua LvYulei Chen
Sep 26, 2020·Frontiers in Pharmacology·Qianqian LiYi Wang
Feb 3, 2020·The Journal of Nutritional Biochemistry·Levi W EvansBradley S Ferguson
Nov 24, 2020·Current Vascular Pharmacology·Denise Coutinho de MirandaMauro César Isoldi

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
FACS
transfection
electrophoresis
PCR
transfections

Software Mentioned

GraphPad
GraphPad Prism

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Adherens Junctions

An adherens junction is defined as a cell junction whose cytoplasmic face is linked to the actin cytoskeleton. They can appear as bands encircling the cell (zonula adherens) or as spots of attachment to the extracellular matrix (adhesion plaques). Adherens junctions uniquely disassemble in uterine epithelial cells to allow the blastocyst to penetrate between epithelial cells. Discover the latest research on adherens junctions here.

Cadherins and Catenins

Cadherins (named for "calcium-dependent adhesion") are a type of cell adhesion molecule (CAM) that is important in the formation of adherens junctions to bind cells with each other. Catenins are a family of proteins found in complexes with cadherin cell adhesion molecules of animal cells: alpha-catenin can bind to β-catenin and can also bind actin. β-catenin binds the cytoplasmic domain of some cadherins. Discover the latest research on cadherins and catenins here.

Related Papers

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Bing XingZai-Wei Zhang
Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Xiaohong ZhangHong Liu
© 2022 Meta ULC. All rights reserved