Emp is a component of the nuclear matrix of mammalian cells and undergoes dynamic rearrangements during cell division

Biochemical and Biophysical Research Communications
Shashi BalaManjit Hanspal

Abstract

Emp, originally detected in erythroblastic islands, is expressed in numerous cell types and tissues suggesting a functionality not limited to hematopoiesis. To study the function of Emp in non-hematopoietic cells, an epitope-tagged recombinant human Emp was expressed in HEK cells. Preliminary studies revealed that Emp partitioned into both the nuclear and Triton X-100-insoluble cytoskeletal fractions in approximately a 4:1 ratio. In this study, we report investigations of Emp in the nucleus. Sequential extractions of interphase nuclei showed that recombinant Emp was present predominantly in the nuclear matrix. Immunofluorescence microscopy showed that Emp was present in typical nuclear speckles enriched with the spliceosome assembly factor SC35 and partially co-localized with actin staining. Coimmunoprecipitation and GST-pull-down assays confirmed the apparent close association of Emp with nuclear actin. During mitosis, Emp was detected at the mitotic spindle/spindle poles, as well as in the contractile ring during cytokinesis. These results suggest that Emp undergoes dynamic rearrangements within the nuclear architecture that are correlated with cell division.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 1, 1977·Cell·T G Clark, R W Merriam
Jul 25, 1975·Science·R Berezney, D S Coffey
Feb 23, 1990·Biochimica Et Biophysica Acta·L KoehlerM Goppelt-Struebe
Mar 1, 1990·The Journal of Cell Biology·D C HeS Penman
Sep 1, 1985·Cell Structure and Function·H Nakayasu, K Ueda
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J K Franz, W W Franke
Jun 1, 1989·Molecular and Cellular Biology·D ChelskyG Jonak
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M BerriosP A Fisher
Jan 1, 1987·Molecular and Cellular Biology·S ZeitlinA Efstratiadis
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·D Z FisherG Blobel
Mar 1, 1971·Experimental Cell Research·M M PerryN S Thomas
Jun 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·T E Schroeder
Jun 1, 1970·Experimental Cell Research·E Skutelsky, D Danon
Jun 1, 1967·The Journal of Cell Biology·E Skutelsky, D Danon
Oct 1, 1967·The Journal of Cell Biology·C F Simpson, J M Kling
Jan 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P C HentzenI Bekhor
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·N SahyounP Cuatrecasas
Jan 1, 1984·Journal of Cell Science. Supplement·L GeraceM Benson
Jan 1, 1981·Cell and Tissue Research·E A Repasky, B S Eckert
Feb 1, 1980·Cell·D M PardollD S Coffey
Jan 1, 1994·Cell Motility and the Cytoskeleton·C ZengB R Brinkley
Jul 4, 1997·The Journal of Biological Chemistry·G NowakP de Lanerolle
Aug 1, 1997·American Journal of Respiratory Cell and Molecular Biology·R PasulaW J Martin
Jan 29, 2000·Critical Reviews in Eukaryotic Gene Expression·J Gotzmann, R Foisner
Oct 13, 2000·Science·L Pestic-DragovichP de Lanerolle
Jun 5, 2002·Annual Review of Biochemistry·Ivan A OlaveGerald R Crabtree
Feb 5, 2003·The Journal of Biological Chemistry·Annmarie PendletonAnna Koffer
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sharon Wald KraussRebecca Heald

❮ Previous
Next ❯

Citations

Oct 30, 2008·Journal of Proteomics·Jakob AlbrethsenConnie R Jimenez
Feb 7, 2015·PloS One·Louisa M SalemiCaroline Schild-Poulter
Aug 16, 2016·Blood Cells, Molecules & Diseases·Gulnaz T JavanShivani Soni
Feb 20, 2008·Current Topics in Developmental Biology·Deepa Manwani, James J Bieker
May 19, 2006·The Journal of Biological Chemistry·Shivani SoniManjit Hanspal
Oct 19, 2019·Journal of Translational Medicine·Shi ZhangHaibo Qiu
Oct 17, 2017·European Journal of Haematology·Gulnaz T JavanShivani Soni

❮ Previous
Next ❯

Related Concepts

Related Feeds

Alternative splicing

Alternative splicing a regulated gene expression process that allows a single genetic sequence to code for multiple proteins. Here is that latest research.