EmPis-1L, an Effective Antimicrobial Peptide Against the Antibiotic-Resistant VBNC State Cells of Pathogenic Bacteria

Probiotics and Antimicrobial Proteins
Bochao HuLi Deng

Abstract

The antibiotic-resistant viable but non-culturable (VBNC) pathogenic bacteria are considered as a new threat to public health. Antimicrobial peptides (AMPs), possessing bactericidal effects in a rapid membrane attacking mode, are supposed to be effective against bacteria entering the VBNC state. In the current study, the activity of grouper AMP piscidin killing the VBNC state cells of pathogenic bacteria Escherichia coli O157, Staphylococcus aureus, and Vibrio parahaemolyticus OS4 was studied. After entering the VBNC state, cells of E. coli O157, S. aureus, and V. parahaemolyticus OS4 developed resistance to the antibiotics Ampicillin and Kanamycin. Rather than truncated form of Malabar grouper piscidin 1 (EmPis-1S), full-length Malabar grouper piscidin 1 (EmPis-1L) showed strong activity to kill the above VBNC bacteria. The VBNC state cells (1 × 105 CFU/mL) of the three species of bacteria could be totally lysed by 10 μmol/L of EmPis-1L in 1 h. The VBNC state cells of S. aureus were most susceptible to EmPis-1L, which killed the cells by 100% in 30 min at the low concentration of 2.0 μmol/L. In addition, EmPis-1L at the concentration of no more than 10 μmol/L showed no observed toxicity to human lung carcinoma epithelial cells...Continue Reading

References

Nov 20, 2001·Nature·U Silphaduang, E J Noga
Jun 7, 2003·Drug News & Perspectives·Edward J Noga, Umaporn Silphaduang
Oct 29, 2003·International Journal of Food Microbiology·W BaffoneG Donelli
Dec 13, 2006·Nature Biotechnology·Robert E W Hancock, Hans-Georg Sahl
Dec 26, 2006·Applied and Environmental Microbiology·Meng DuYingeng Wang
Dec 28, 2006·Diseases of Aquatic Organisms·U SilphaduangE J Noga
May 1, 2008·Biochemical and Biophysical Research Communications·Woo Sang SungDong Gun Lee
Sep 9, 2009·International Journal of Food Microbiology·Chiou-Jour LaiHin-Chung Wong
Jan 12, 2010·FEMS Microbiology Reviews·James D Oliver
Dec 24, 2010·Virulence·Jochen Wiesner, Andreas Vilcinskas
Dec 12, 2012·FEMS Microbiology Ecology·Joanna Nowakowska, James D Oliver
Jan 8, 2013·International Journal of Food Microbiology·Chao-Ping SuHin-chung Wong
Mar 6, 2013·Expert Review of Anti-infective Therapy·Jack N PendletonBrendan F Gilmore
Nov 26, 2013·European Journal of Medicinal Chemistry·Diego FacconePaulo Cesar Maffía
Mar 1, 2014·Environmental Toxicology and Pharmacology·Fude WangYongji Yang
Aug 19, 2014·Frontiers in Public Health·Thandavarayan RamamurthySumio Shinoda
Jan 15, 2015·Environmental Science & Technology·Shenghua ZhangXin Yu
Mar 12, 2015·Current Medicinal Chemistry·Stefania GaldieroMassimiliano Galdiero
May 18, 2016·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·D I AnderssonJ Z Kubicek-Sutherland
Aug 16, 2016·Microbiological Research·Alessandra C RiosVictor M Balcão
Dec 16, 2016·Journal of Microbiology and Biotechnology·Tian DingDonghong Liu
Apr 20, 2017·Frontiers in Microbiology·Xihong ZhaoTian Ding
Jul 12, 2017·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Pooi Yin Chung, Ramona Khanum

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