EMPTY PERICARP16 is required for mitochondrial nad2 intron 4 cis-splicing, complex I assembly and seed development in maize

The Plant Journal : for Cell and Molecular Biology
Zhi-Hui XiuBao-Cai Tan

Abstract

In higher plants, chloroplast and mitochondrial transcripts contain a number of group II introns that need to be precisely spliced before translation into functional proteins. However, the mechanism of splicing and the factors involved in this process are not well understood. By analysing a seed mutant in maize, we report here the identification of Empty pericarp16 (Emp16) that is required for splicing of nad2 intron 4 in mitochondria. Disruption of Emp16 function causes developmental arrest in the embryo and endosperm, giving rise to an empty pericarp phenotype in maize. Differentiation of the basal endosperm transfer layer cells is severely affected. Molecular cloning indicates that Emp16 encodes a P-type pentatricopeptide repeat (PPR) protein with 11 PPR motifs and is localized in the mitochondrion. Transcript analysis revealed that mitochondrial nad2 intron 4 splicing is abolished in the emp16 mutants, leading to severely reduced assembly and activity of complex I. In response, the mutant dramatically increases the accumulation of mitochondrial complex III and the expression of alternative oxidase AOX2. These results imply that EMP16 is specifically required for mitochondrial nad2 intron 4 cis-splicing and is essential for ...Continue Reading

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Citations

Mar 28, 2017·The Plant Journal : for Cell and Molecular Biology·Manjun CaiFazhan Qiu
May 13, 2017·The International Journal of Biochemistry & Cell Biology·Matthew NguLinda Bonen
Aug 2, 2017·Proceedings of the National Academy of Sciences of the United States of America·Damien GuillaumotClaire Lurin
Mar 27, 2018·Physiologia Plantarum·Wei-Qing WangSong-Quan Song
Nov 13, 2018·The Plant Journal : for Cell and Molecular Biology·Jiaqiang DongJoachim Messing
May 12, 2019·Plant & Cell Physiology·Zhenjing RenYunjun Liu
Apr 6, 2019·The Plant Genome·Adam VanousThomas Lübberstedt
Jul 25, 2019·Journal of Integrative Plant Biology·Hong-Chun WangBao-Cai Tan
Nov 24, 2017·G3 : Genes - Genomes - Genetics·Susan Gabay-LaughnanChristine D Chase
Oct 6, 2017·Journal of Integrative Plant Biology·Xiaojie LiJinsheng Lai
Jun 26, 2020·Frontiers in Plant Science·Huanhuan YangBao-Cai Tan
Jan 3, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Peng ZhengJumin Tu

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