PMID: 9182758May 30, 1997Paper

Empty site forms of the SRP54 and SR alpha GTPases mediate targeting of ribosome-nascent chain complexes to the endoplasmic reticulum

Cell
P J Rapiejko, R Gilmore

Abstract

The SRP54 and SR alpha subunits of the signal recognition particle (SRP) and the SRP receptor (SR) undergo a tightly coupled GTPase cycle that mediates the signal sequence-dependent attachment of ribosomes to the Sec61 complex. Here, we show that SRP54 and SR alpha are in the empty site conformation prior to contact between the SRP-ribosome complex and the membrane-bound SR. Cooperative binding of GTP to SRP54 and SR alpha stabilizes the SRP-SR complex and initiates signal sequence transfer from SRP54 to Sec61 alpha. The GTP-bound conformations of SR alpha and SRP54 perform distinct roles, with SR alpha performing a predominant role in complex stabilization. Hydrolysis by both SRP54 and SR alpha is a prerequisite for dissociation of the SRP-SR complex.

References

Apr 1, 1991·The Journal of Cell Biology·S HighB Dobberstein
May 3, 1991·Cell·S M Simon, G Blobel
Nov 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·U C KriegA E Johnson
Jan 1, 1994·Annual Review of Cell Biology·P Walter, A E Johnson
Dec 1, 1994·Molecular and Cellular Biology·S M AlthoffJ A Wise
Aug 1, 1994·The Journal of Cell Biology·K U KaliesT A Rapoport

❮ Previous
Next ❯

Citations

May 16, 2002·Biochimica Et Biophysica Acta·Irina V Shepotinovskaya, Douglas M Freymann
Feb 13, 2003·Current Opinion in Structural Biology·A Elisabeth Sauer-Eriksson, Tobias Hainzl
Feb 13, 2002·Current Opinion in Structural Biology·Klemens WildStephen Cusack
Mar 10, 2001·Trends in Microbiology·J Eichler, R Moll
Aug 23, 2003·Trends in Biochemical Sciences·Nathan N Alder, Steven M Theg
Apr 2, 1999·European Journal of Biochemistry·O J GrussB Dobberstein
Oct 22, 2008·Acta Crystallographica. Section D, Biological Crystallography·Ursula D RamirezDouglas M Freymann
Jan 17, 2004·Science·Pamela J FociaDouglas M Freymann
Jun 8, 2001·Annual Review of Biochemistry·R J KeenanP Walter
Jun 11, 2004·Annual Review of Biochemistry·Jennifer A Doudna, Robert T Batey
Dec 28, 1999·Annual Review of Cell and Developmental Biology·A E Johnson, M A van Waes
Sep 13, 2005·Annual Review of Microbiology·Joen LuirinkJan-Willem de Gier
Jun 30, 2009·Biological Chemistry·Przemyslaw GrudnikIrmgard Sinning
Aug 19, 2007·Proceedings of the National Academy of Sciences of the United States of America·Gert BangeIrmgard Sinning
Mar 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Shu-ou Shan, Peter Walter
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·N ZerangueB Schwappach
Jul 31, 2010·Archaea : an International Microbiological Journal·Christian Zwieb, Shakhawat Bhuiyan
Sep 5, 2001·FEBS Letters·V Goder, M Spiess
May 6, 2009·Journal of Molecular Biology·David BraigHans-Georg Koch
Dec 23, 2006·Journal of Structural Biology·Joseph Gawronski-Salerno, Douglas M Freymann
Dec 14, 2005·Journal of Structural Biology·Talal GarianiA Elisabeth Sauer-Eriksson
Dec 30, 2003·Proteins·Pamela J FociaDouglas M Freymann
Mar 10, 2007·EMBO Reports·Yaniv LustigShulamit Michaeli
Dec 29, 2011·Biochimica Et Biophysica Acta·Joen LuirinkJan-Willem de Gier
Mar 28, 2013·Critical Reviews in Biochemistry and Molecular Biology·Tslil Ast, Maya Schuldiner
Apr 26, 2014·Molecular Membrane Biology·Kärt DenksHans-Georg Koch
Oct 21, 1998·Current Biology : CB·H D Bernstein
Mar 14, 2013·Biochimica Et Biophysica Acta·Yvonne NyathiMartin R Pool

❮ Previous
Next ❯

Related Concepts

Related Feeds

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.

Blood And Marrow Transplantation

The use of hematopoietic stem cell transplantation or blood and marrow transplantation (bmt) is on the increase worldwide. BMT is used to replace damaged or destroyed bone marrow with healthy bone marrow stem cells. Here is the latest research on bone and marrow transplantation.