EMT circulating tumor cells detected by cell-surface vimentin are associated with prostate cancer progression

Oncotarget
Arun SatelliShulin Li

Abstract

Recent advances in the field of circulating tumor cells (CTC) have shown promise in this liquid biopsy-based prognosis of patient outcome. However, not all of the circulating cells are tumor cells, as evidenced by a lack of tumor-specific markers. The current FDA standard for capturing CTCs (CellSearch) relies on an epithelial marker and cells captured via CellSearch cannot be considered to have undergone EMT. Therefore, it is difficult to ascertain the presence and relevance of any mesenchymal or EMT-like CTCs. To address this gap in technology, we recently discovered the utility of cell-surface vimentin (CSV) as a marker for detecting mesenchymal CTCs from sarcoma, breast, and colon cancer. Here we studied peripheral blood samples of 48 prostate cancer (PCA) patients including hormone sensitive and castration resistant sub-groups. Blood samples were analyzed for three different properties including our own CSV-based CTC enumeration (using 84-1 mAb against CSV), CellSearch-based epithelial CTC counts, and serum prostate-specific antigen (PSA) quantification. Our data demonstrated that in comparison with CellSearch, the CSV-based method had greater sensitivity and specificity. Further, we observed significantly greater numbers ...Continue Reading

References

Aug 20, 2004·The New England Journal of Medicine·Massimo CristofanilliDaniel F Hayes
Jul 2, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Steven J CohenNeal J Meropol
Oct 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Johann S de BonoDerek Raghavan
Mar 20, 2009·The New England Journal of Medicine·Gerald L AndrioleUNKNOWN PLCO Project Team
Jun 3, 2009·The Journal of Clinical Investigation·Raghu Kalluri, Robert A Weinberg
Jun 4, 2011·Cellular and Molecular Life Sciences : CMLS·Arun Satelli, Shulin Li
Jun 22, 2011·Nature Reviews. Urology·Jones T Nauseef, Michael D Henry
Jan 22, 2014·Nature Reviews. Clinical Oncology·Matthew G KrebsCaroline Dive
Jan 23, 2014·Cancer Research·Arun SatelliShulin Li
Feb 22, 2014·Nature Reviews. Molecular Cell Biology·Samy LamouilleRik Derynck
Mar 13, 2014·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Amir GoldkornNicholas J Vogelzang
Mar 13, 2014·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Terence W Friedlander, Lawrence Fong
Mar 22, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·P K GroverJ E Hardingham
Jun 14, 2014·American Journal of Clinical Pathology·Jaime H Noguez, Corinne R Fantz
Dec 18, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Arun SatelliShulin Li
Jan 7, 2015·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 9, 2016·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal

❮ Previous
Next ❯

Citations

Dec 16, 2017·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·L YangC Ni
May 31, 2018·Stem Cells·Sergej SkvortsovAnna Dubrovska
Nov 2, 2018·Genes, Chromosomes & Cancer·Evi Lianidou, Klaus Pantel
Aug 17, 2019·Proceedings of the National Academy of Sciences of the United States of America·Su Bin LimChwee Teck Lim
Sep 8, 2020·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Alison E PattesonPaul A Janmey
Dec 19, 2019·Journal of Hematology & Oncology·Ye ZhangTing Liu
Jul 20, 2020·International Journal of Cancer. Journal International Du Cancer·Isabel HeidrichKlaus Pantel
Sep 6, 2018·Therapeutic Advances in Medical Oncology·Raffaele PalmirottaFranco Silvestris
Mar 25, 2018·Current Oncology Reports·Gareth J Morrison, Amir Goldkorn
Jul 28, 2019·Translational Research : the Journal of Laboratory and Clinical Medicine·Izhar S BatthShulin Li
Jan 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Zhilong ChenJi Ma
Mar 5, 2021·Esophagus : Official Journal of the Japan Esophageal Society·Daisuke UjiieKoji Kono
Mar 7, 2021·International Journal of Molecular Sciences·Izhar Singh BatthAddanki P Kumar
Apr 25, 2021·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Chia-Lin HsuPan-Chyr Yang
Apr 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Tiantian QinCong Wang
Dec 7, 2021·Small·Łukasz SuprewiczAlison E Patteson

❮ Previous
Next ❯

Methods Mentioned

BETA
PCA
flow cytometry
fluorescence
confocal microscopy
biopsies
biopsy
blood collection

Software Mentioned

GraphPad
lme4
CellSearch
LSM5
CSV
Prism
R

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.