Emulsions Made of Oils from Seeds of GM Flax Protect V79 Cells against Oxidative Stress

Oxidative Medicine and Cellular Longevity
Katarzyna Skórkowska-TelichowskaKazimierz Gąsiorowski

Abstract

Polyunsaturated fatty acids, sterols, and hydrophilic phenolic compounds are components of flax oil that act as antioxidants. We investigated the impact of flax oil from transgenic flax in the form of emulsions on stressed Chinese hamster pulmonary fibroblasts. We found that the emulsions protect V79 cells against the H2O2 and the effect is dose dependent. They reduced the level of intracellular reactive oxygen species and protected genomic DNA against damage. The rate of cell proliferation increased upon treatment with the emulsions at a low concentration, while at a high concentration it decreased significantly, accompanied by increased frequency of apoptotic cell death. Expression analysis of selected genes revealed the upregulatory impact of the emulsions on the histones, acetylases, and deacetylases. Expression of apoptotic, proinflammatory, and anti-inflammatory genes was also altered. It is thus suggested that flax oil emulsions might be useful as a basis for biomedical products that actively protect cells against inflammation and degeneration. The beneficial effect on fibroblast resistance to oxidative damage was superior in the emulsion made of oil from transgenic plants which was correlated with the quantity of antiox...Continue Reading

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Citations

Jun 9, 2018·Molecular Nutrition & Food Research·José M Lou-BonafonteJesús Osada
Feb 6, 2020·Pharmacological Reports : PR·Tomasz GębarowskiKazimierz Gąsiorowski

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Datasets Mentioned

BETA
X04080
X14589
X15537
AY033489

Methods Mentioned

BETA
environmental stresses
glycosylation
transgenic
electrophoresis
PCR

Software Mentioned

CometPlus
Chemstation

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